| Title: | The glucanase-soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae |
| Author(s): | de Nobel JG; Klis FM; Priem J; Munnik T; van den Ende H; |
| Address: | "Department of Molecular Cell Biology, University of Amsterdam, The Netherlands" |
| ISSN/ISBN: | 0749-503X (Print) 0749-503X (Linking) |
| Abstract: | "The cell wall porosity of batch-grown Saccharomyces cerevisiae was maximal in the early exponential phase and fell off rapidly to lower levels in later growth phases. Treatment of stationary-phase cells with alpha-mannosidase restored wall porosity to the level of cells in early exponential phase. When cells in the early exponential phase were treated with alpha-mannosidase, or tunicamycin, an inhibitor of N-glycosylation, even higher porosities were obtained. Mutants with truncated mannan side-chains in their wall proteins also had very porous walls. The importance of the mannan side-chains for wall porosity was also seen during sexual induction. Treatment with alpha pheromone, which leads to the formation of wall proteins with shorter mannan side-chains, enhanced wall porosity. Disulphide bridges also affect cell wall porosity. They were predominantly found in the glucanase-soluble wall proteins. Because the main part of the mannan side-chains is also found in this family of wall proteins, our results demonstrate that the glucanase-soluble mannoproteins limit cell wall porosity in yeast" |
| Keywords: | Benzenesulfonates/pharmacology Cell Wall/drug effects/metabolism/ultrastructure DEAE-Dextran/pharmacology Disulfides/metabolism Hydrolases/pharmacology Mannosidases/*metabolism Membrane Glycoproteins/*metabolism Porosity Saccharomyces cerevisiae/drug effe; |
| Notes: | "Medlinede Nobel, J G Klis, F M Priem, J Munnik, T van den Ende, H eng England 1990/11/01 Yeast. 1990 Nov-Dec; 6(6):491-9. doi: 10.1002/yea.320060606" |
