« Previous AbstractBiochemical crypsis in the avoidance of natural enemies by an insect herbivore    Next AbstractIdentification of a ligand-binding site in an immunoglobulin fold domain of the Saccharomyces cerevisiae adhesion protein alpha-agglutinin »

Mol Gen Genet

Title:		Genetics of a-agglutunin function in Saccharomyces cerevisiae
Author(s):		de Nobel H; Pike J; Lipke PN; Kurjan J;
Address:		"Department of Microbiology and Molecular Genetics, University of Vermont, College of Medicine 05405-0068, USA"
Journal Title:		Mol Gen Genet
Year:		1995
Volume:		247
Issue:		4
Page Number:		409 - 415
DOI:		10.1007/BF00293141
ISSN/ISBN:		0026-8925 (Print) 0026-8925 (Linking)
Abstract:		"The Saccharomyces cerevisiae cell adhesion protein a-agglutinin is composed of an anchorage subunit (Aga1p) and an adhesion subunit (Aga2p). Although functional a-agglutinin is expressed only by a cells, previous results indicated that AGA1 RNA is expressed in both a and alpha cells after pheromone induction. Expression of the Aga2p adhesion subunit in alpha cells allowed a-agglutinability, indicating that alpha cells express the a-agglutinin anchorage subunit, although no role for Aga1p in alpha cells has been identified. Most of the a-specific agglutination-defective mutants isolated previously were defective in AGA1; a single mutant (La199) was a candidate for an aga2 mutant. Expression of AGA2 under PGK control allowed secretion of active Aga2p from control strains but did not complement the La199 agglutination defect or allow secretion of Aga2p from La199, suggesting that the La199 mutation might identify a new gene required for a-agglutinin function. However, the La199 agglutination defect showed tight linkage to aga2::URA3 and did not complement aga2::URA3 in a/a diploids. The aga2 gene cloned from La199 was nonfunctional and contained an ochre mutation. The inability of pPGK-AGA2 to express functional Aga2p in La199 was shown to result from an additional mutation(s) that reduces expression of plasmid-borne genes. AGA2 was mapped to the left arm of chromosome VII approximately 28 cM from the centromere"
Keywords:		"Base Sequence Cell Adhesion Molecules/genetics Chromosome Mapping DNA Primers/chemistry Fungal Proteins/*genetics Genes, Fungal Genetic Complementation Test Mating Factor Molecular Sequence Data Peptides/*genetics Saccharomyces cerevisiae/*genetics *Sacch;"
Notes:		"Medlinede Nobel, H Pike, J Lipke, P N Kurjan, J eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Germany 1995/05/20 Mol Gen Genet. 1995 May 20; 247(4):409-15. doi: 10.1007/BF00293141"