« Previous Abstract"The effect of chemical information on the spatial distribution of fruit flies: II Parameterization, calibration, and sensitivity"    Next AbstractIndoor and outdoor monitoring of volatile organic compounds in school buildings: indicators based on health risk assessment to single out critical issues »

Anal Bioanal Chem

Title:		Chemical characterization of exhaled breath to differentiate between patients with malignant plueral mesothelioma from subjects with similar professional asbestos exposure
Author(s):		de Gennaro G; Dragonieri S; Longobardi F; Musti M; Stallone G; Trizio L; Tutino M;
Address:		"Department of Chemistry, University of Bari Aldo Moro, 70126 Bari, Italy"
Journal Title:		Anal Bioanal Chem
Year:		2010
Volume:		20101006
Issue:		7-Aug
Page Number:		3043 - 3050
DOI:		10.1007/s00216-010-4238-y
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"Malignant pleural mesothelioma (MPM) is an aggressive tumour whose main aetiology is the long-term exposure to asbestos fibres. The diagnostic procedure of MPM is difficult and often requires invasive approaches; therefore, it is clinically important to find accurate markers for MPM by new noninvasive methods that may facilitate the diagnostic process and identify patients at an earlier stage. In the present study, the exhaled breath of 13 patients with histology-established diagnosis of MPM, 13 subjects with long-term certified professional exposure to asbestos (EXP) and 13 healthy subjects without exposure to asbestos (healthy controls, HC) were analysed. An analytical procedure to determine volatile organic compounds by sampling of air on a bed of solid sorbent and thermal desorption GC-MS analysis was developed in order to identify the compounds capable of discriminating among the three groups. The application of univariate (ANOVA) and multivariate statistical treatments (PCA, DFA and CP-ANN) showed that cyclopentane and cyclohexane were the dominant variables able to discriminate among the three groups. In particular, it was found that cyclohexane is the only compound able to differentiate the MPM group from the other two; therefore, it can be a possible marker of MPM. Cyclopentane is the dominant compound in the discrimination between EXP and the other groups (MPM and HC); then, it can be considered a good indicator for long-term asbestos exposure. This result suggests the need to perform frequent and thorough investigations on people exposed to asbestos in order to constantly monitor their state of health or possibly to study the evolution of disease over time"
Keywords:		"Aged Aged, 80 and over Asbestos/*poisoning Breath Tests/*methods Case-Control Studies Cross-Sectional Studies Discriminant Analysis Gas Chromatography-Mass Spectrometry/methods Humans Mesothelioma/diagnosis/etiology/*metabolism Middle Aged Neural Networks;"
Notes:		"Medlinede Gennaro, G Dragonieri, S Longobardi, F Musti, M Stallone, G Trizio, L Tutino, M eng Germany 2010/10/07 Anal Bioanal Chem. 2010 Dec; 398(7-8):3043-50. doi: 10.1007/s00216-010-4238-y. Epub 2010 Oct 6"