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Environ Microbiol

Title:		Involvement of thermophilic archaea in the biocorrosion of oil pipelines
Author(s):		Davidova IA; Duncan KE; Perez-Ibarra BM; Suflita JM;
Address:		"Department of Botany and Microbiology and OU Biocorrosion Center, University of Oklahoma, Norman, OK, USA"
Journal Title:		Environ Microbiol
Year:		2012
Volume:		20120319
Issue:		7
Page Number:		1762 - 1771
DOI:		10.1111/j.1462-2920.2012.02721.x
ISSN/ISBN:		1462-2920 (Electronic) 1462-2912 (Linking)
Abstract:		"Two thermophilic archaea, strain PK and strain MG, were isolated from a culture enriched at 80 degrees C from the inner surface material of a hot oil pipeline. Strain PK could ferment complex organic nitrogen sources (e.g. yeast extract, peptone, tryptone) and was able to reduce elemental sulfur (S degrees ), Fe(3+) and Mn(4+) . Phylogenetic analysis revealed that the organism belonged to the order Thermococcales. Incubations of this strain with elemental iron (Fe degrees ) resulted in the abiotic formation of ferrous iron and the accumulation of volatile fatty acids during yeast extract fermentation. The other isolate, strain MG, was a H(2) :CO(2) -utilizing methanogen, phylogenetically affiliated with the genus Methanothermobacter family. Co-cultures of the strains grew as aggregates that produced CH(4) without exogenous H(2) amendment. The co-culture produced the same suite but greater concentrations of fatty acids from yeast extract than did strain PK alone. Thus, the physiological characteristics of organisms both alone and in combination could conceivably contribute to pipeline corrosion. The Thermococcus strain PK could reduce elemental sulfur to sulfide, produce fatty acids and reduce ferric iron. The hydrogenotrophic methanogen strain MG enhanced fatty acid production by fermentative organisms but could not couple the dissolution Fe degrees with the consumption of water-derived H(2) like other methanogens"
Keywords:		"Archaea/genetics/*metabolism Coculture Techniques Corrosion DNA, Archaeal/genetics Fatty Acids/biosynthesis Fermentation Ferric Compounds/*metabolism Hot Temperature Methane/biosynthesis Petroleum/*microbiology Phylogeny RNA, Ribosomal, 16S/genetics Seque;"
Notes:		"MedlineDavidova, Irene A Duncan, Kathleen E Perez-Ibarra, B Monica Suflita, Joseph M eng Research Support, Non-U.S. Gov't England 2012/03/21 Environ Microbiol. 2012 Jul; 14(7):1762-71. doi: 10.1111/j.1462-2920.2012.02721.x. Epub 2012 Mar 19"