| Title: | Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe |
| Address: | "School of Biochemistry, University of Birmingham, UK" |
| ISSN/ISBN: | 0172-8083 (Print) 0172-8083 (Linking) |
| Abstract: | "Investigations into sexual differentiation and pheromone response in the fission yeast Schizosaccharomyces pombe are complicated by the need to first starve the cells of nitrogen. Most mating-related experiments are therefore performed on non-dividing cells. Here we overcome this problem by using two mutants that bypass the nutritional requirements and respond to the M-factor mating pheromone in rich medium. The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP. When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state derepressed for pheromone-controlled functions. We now report that an h+ pat1-114 strain growing mitotically at 23 degrees C responds to M-factor. This shows that the pat1 protein kinase can be tuned to derepress nutritional signalling while repressing the other stages in the differentiation process" |
| Keywords: | "Adenylyl Cyclases/biosynthesis/genetics Cell Division Crosses, Genetic G1 Phase *Genes, Fungal Kinetics Mating Factor Mutagenesis Peptides/*genetics/physiology Pheromones/genetics Schizosaccharomyces/cytology/*genetics Time Factors Transcription, Genetic;" |
| Notes: | "MedlineDavey, J Nielsen, O eng Research Support, Non-U.S. Gov't 1994/08/01 Curr Genet. 1994 Aug; 26(2):105-12. doi: 10.1007/BF00313796" |
