« Previous AbstractHS-GC-IMS Analysis of Volatile Organic Compounds in Different Varieties and Harvesting Times of Rhizoma gastrodiae (Tian Ma) in Yunnan Province    Next AbstractImproving the Antioxidant Activity and Flavor of Faba (Vicia faba L.) Leaves by Domestic Cooking Methods »

Biochem Biophys Res Commun

Title:		Novel female-specific trans-spliced and alternative splice forms of dsx in the silkworm Bombyx mori
Author(s):		Duan J; Xu H; Wang F; Ma S; Zha X; Guo H; Zhao P; Xia Q;
Address:		"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China"
Journal Title:		Biochem Biophys Res Commun
Year:		2013
Volume:		20121220
Issue:		3
Page Number:		630 - 635
DOI:		10.1016/j.bbrc.2012.12.062
ISSN/ISBN:		1090-2104 (Electronic) 0006-291X (Linking)
Abstract:		"The Bombyx mori doublesex gene (Bmdsx) plays an important role in somatic sexual development. Its pre-mRNA splices in a sex-specific manner to generate two female-specific and one male-specific splice forms. The present study investigated six novel dsx variants generated by trans-splicing between female dsx transcripts and two additional novel genes, dsr1 and dsr2. Expression analysis indicated that Bmdsx-dsr1 represented splicing noise, whereas dsr2, which trans-spliced with dsx to generate five variants, regulated the expression of the female-specific B. mori dsx transcript Bmdsx(F)s. We unexpectedly found a novel exon 2n insertion during Bmdsx transcription, which did not influence the validity of the novel protein, BmDSX(F3). Ectopic expression of BmDSX(F3) repressed the pheromone-binding protein gene and the testis-specific gene A2 in males, and activated of the storage protein 1 gene. Our findings suggest that trans-splicing is a novel regulatory function of Bmdsx, which participates in female sexual development by regulating the expression of three BmDSX(F) proteins"
Keywords:		"*Alternative Splicing Animals Animals, Genetically Modified/genetics/metabolism Bombyx/*genetics/metabolism Carrier Proteins/genetics DNA-Binding Proteins/*genetics/metabolism Female Insect Proteins/*genetics/metabolism Intercellular Signaling Peptides an;"
Notes:		"MedlineDuan, Jianping Xu, Hanfu Wang, Feng Ma, Sanyuan Zha, Xingfu Guo, Huizhen Zhao, Ping Xia, Qingyou eng Research Support, Non-U.S. Gov't 2012/12/25 Biochem Biophys Res Commun. 2013 Feb 15; 431(3):630-5. doi: 10.1016/j.bbrc.2012.12.062. Epub 2012 Dec 20"