| Title: | "Characterization and function analysis of a novel gene, Hc-maoc-1, in the parasitic nematode Haemonochus contortus" |
| Author(s): | Ding H; Shi H; Shi Y; Guo X; Zheng X; Chen X; Zhou Q; Yang Y; Du A; |
| Address: | "College of Animal Sciences, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211, China. College of Animal Sciences, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. yangyi0607@zju.edu.cn. College of Animal Sciences, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China. afdu@zju.edu.cn" |
| DOI: | 10.1186/s13071-017-1991-1 |
| ISSN/ISBN: | 1756-3305 (Electronic) 1756-3305 (Linking) |
| Abstract: | "BACKGROUND: Enoyl-CoA hydratase (MAOC) is required for the biosynthesis of the fatty acid-derive side chains of the ascaroside via peroxisome beta-oxidation in the free-living nematode Caenorhabditis elegans. The derivative of dideoxy-sugar, ascarylose is used as dauer pheromones or daumones to induce development of the stress-resistant dauer larvae stage. METHODS: Hc-maoc-1 gene was obtained by searching the Wellcome Trusts Sanger Institute's H. contortus genomic database. qRT-PCR was performed to analyse the transcriptional levels of Hc-maoc-1 with different developmental stages as templates. IFA was carried out to determine the expression pattern in L3 larvae and micro-injection was used to verify the promoter activity of 5'-flanking region of Hc-maoc-1. Overexpression and RNAi experiments were applied in N(2) strain to ascertain the gene function of Hc-maoc-1. RESULTS: The full-length cDNA of Hc-maoc-1 was 900 bp in length, which contained eight exons separated by seven introns and possessed the Hotdog domain and the MaoC-like domain, together with several other residues and a hydratase 2 motif. It was transcribed throughout the lifecycle and peaked in the fourth-stage larvae (L4) of H. contortus; however, its transcription level decreased in diapausing L4. The protein expression and location of Hc-MAOC-1 were mainly in the intestine of L3 larvae. Overexpression of Ce-maoc-1 and Hc-maoc-1 in C. elegans showed extended lifespan and increased body size. The protein Ce-MAOC-1 and Hc-MAOC-1 were localized in the intestine with a punctate pattern. In C. elegans, knockdown of Ce-maoc-1 conferred shortened lifespan and body lengths, decreased brood size and increased lipid storage. CONCLUSION: Caenorhabditis elegans was used as a model organism to ascertain the function of Hc-maoc-1 in H. contortus. Our results showed the similar characteristics and functions with Ce-maoc-1 and provided evidences of the potential functions of Hc-maoc-1 in biosynthesis of daumones in H. contortus" |
| Keywords: | "Animals Caenorhabditis elegans/genetics/growth & development Enoyl-CoA Hydratase/*genetics/*metabolism Fluorescent Antibody Technique, Direct Gene Expression Profiling Haemonchus/*enzymology/genetics/*growth & development Real-Time Polymerase Chain Reacti;" |
| Notes: | "MedlineDing, Haojie Shi, Hengzhi Shi, Yu Guo, Xiaolu Zheng, Xiuping Chen, Xueqiu Zhou, Qianjin Yang, Yi Du, Aifang eng Research Support, Non-U.S. Gov't England 2017/02/09 Parasit Vectors. 2017 Feb 6; 10(1):67. doi: 10.1186/s13071-017-1991-1" |
