« Previous AbstractAn Alternative Surgical Treatment of Giant Serpentine Aneurysm of the Middle Cerebral Artery: Resection and End-to-End Anastomosis    Next Abstract"Diversity of native larvivorous fish fauna during pre- and post-tsunami in Car Nicobar, Andaman & Nicobar Islands, India" »

Anal Chem

Title:		Investigation of gender-specific exhaled breath volatome in humans by GCxGC-TOF-MS
Author(s):		Das MK; Bishwal SC; Das A; Dabral D; Varshney A; Badireddy VK; Nanda R;
Address:		"Immunology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India"
Journal Title:		Anal Chem
Year:		2014
Volume:		20131227
Issue:		2
Page Number:		1229 - 1237
DOI:		10.1021/ac403541a
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"Exploring gender-specific metabolic differences in biofluids provides a basic understanding of the physiological and metabolic phenotype of healthy subjects. Many reports have shown gender-specific metabolome profiles in the urine and serum of healthy subjects; however, limited studies focusing on exhaled human breath are available in the literature. In this study, we profiled the exhaled breath (~450 mL) volatile organic compounds (VOCs) of 47 healthy volunteers (age: 19-47; 23 male (M) and 24 female (F)) using a multidimensional gas chromatography and mass spectrometry and employed chemometric analysis to identify gender-specific VOCs. Eleven exhaled breath VOCs were identified from both uni and multivariate analysis from a training set (M = 15, F = 15) that could differentiate the genders within a healthy population. A partial least-squares discriminate analysis (PLS-DA) model built using these putative markers showed high accuracy in predicting (area under the receiver operating characteristic curve >0.9) a hold out/test sample set (n = 17). The outcomes of this report open up new avenues to undertake larger studies to elucidate the association of exhaled breath metabolites with gender-specific disease phenotypes and pharmacokinetics in the future"
Keywords:		Adult Analysis of Variance Biomarkers/analysis Breath Tests Discriminant Analysis Exhalation/*physiology Female Gas Chromatography-Mass Spectrometry Healthy Volunteers Humans Least-Squares Analysis Male *Metabolome Middle Aged ROC Curve Sex Factors Spectr;
Notes:		"MedlineDas, Mrinal Kumar Bishwal, Subasa Chandra Das, Aleena Dabral, Deepti Varshney, Ankur Badireddy, Vinod Kumar Nanda, Ranjan eng Research Support, Non-U.S. Gov't 2013/12/20 Anal Chem. 2014 Jan 21; 86(2):1229-37. doi: 10.1021/ac403541a. Epub 2013 Dec 27"