| Title: | "Cytochrome P450s from the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae): Expression profiles of different stages and responses to host allelochemicals" |
| Author(s): | Dai L; Ma M; Wang C; Shi Q; Zhang R; Chen H; |
| Address: | "College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, China. College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address: chenhui@nwsuaf.edu.cn" |
| DOI: | 10.1016/j.ibmb.2015.08.004 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Bark beetles oxidize the defensive allelochemicals from their host trees to both detoxify and convert these materials into components of their pheromone system. The ability of this insect to adapt to its chemical environment might be explained by the action of major detoxification enzymes such as cytochrome P450s (or CYPs). Sixty-four sequences coding for P450s were identified, and most of the transcripts were found to be expressed in the larvae, pupae and adults of Dendroctonus armandi. To gain information on how these genes help D. armandi overcome the host defense, differential transcript levels of the CYP genes were observed between sexes and within the sexes. Significant differences were observed among developmental stages, in feeding on the phloem of Pinus armandi and in exposure to stimuli ((+/-)-alpha-pinene, (S)-(-)-alpha-pinene, (S)-(-)-beta-pinene, (+)-3-carene, (+/-)-limonene and turpentine oil) for 8 h. We investigated the effect of sex and generations on the survivorship of individual D. armandi that were exposed to host volatiles at concentrations comparable to constitutive and induced levels of defense using fumigant exposure to understand the ability of the beetles to tolerate host defensive chemicals. The differential transcript accumulation patterns of CYP genes of these bark beetle provided insight into the ecological interactions of D. armandi with its host pine" |
| Keywords: | "Animals China Cytochrome P-450 Enzyme System/*genetics/metabolism Female Genes, Insect Larva/enzymology/genetics Male Monoterpenes/*metabolism Pheromones/*metabolism Phloem Pinus/*parasitology Pupa/enzymology/genetics Weevils/genetics/growth & development;" |
| Notes: | "MedlineDai, Lulu Ma, Mingyuan Wang, Chunyan Shi, Qi Zhang, Ranran Chen, Hui eng Research Support, Non-U.S. Gov't England 2015/09/01 Insect Biochem Mol Biol. 2015 Oct; 65:35-46. doi: 10.1016/j.ibmb.2015.08.004. Epub 2015 Aug 28" |
