| Title: | Diversity and technological potential of lactic acid bacteria of wheat flours |
| Author(s): | Alfonzo A; Ventimiglia G; Corona O; Di Gerlando R; Gaglio R; Francesca N; Moschetti G; Settanni L; |
| Address: | "Department of Agricultural and Forestry Science, University of Palermo, Palermo, Italy" |
| ISSN/ISBN: | 1095-9998 (Electronic) 0740-0020 (Linking) |
| Abstract: | "Lactic acid bacteria (LAB) were analysed from wheat flours used in traditional bread making throughout Sicily (southern Italy). Plate counts, carried out in three different media commonly used to detect food and sourdough LAB, revealed a maximal LAB concentration of approximately 4.75 Log CFU g(-1). Colonies representing various morphological appearances were isolated and differentiated based on phenotypic characteristics and genetic analysis by randomly amplified polymorphic DNA (RAPD)-PCR. Fifty unique strains were identified. Analysis by 16S rRNA gene sequencing grouped the strains into 11 LAB species, which belonged to six genera: Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Weissella. Weissella cibaria, Lactobacillus plantarum, Leuconostoc pseudomesenteroides and Leuconostoc citreum were the most prevalent species. The strains were not geographically related. Denaturing gradient gel electrophoresis (DGGE) analysis of total DNA of flour was used to provide a more complete understanding of the LAB population; it confirmed the presence of species identified with the culture-dependent approach, but did not reveal the presence of any additional LAB species. Finally, the technological characteristics (acidifying capacity, antimicrobial production, proteolytic activity, organic acid, and volatile organic compound generation) of the 50 LAB strains were investigated. Eleven strains were selected for future in situ applications" |
| Keywords: | Acids/metabolism Bacterial Proteins/metabolism *Biodiversity Flour/*microbiology Industrial Microbiology Lactic Acid/metabolism Lactobacillaceae/classification/genetics/*isolation & purification/*metabolism Peptide Hydrolases/metabolism Phylogeny Triticum; |
| Notes: | "MedlineAlfonzo, Antonio Ventimiglia, Giusi Corona, Onofrio Di Gerlando, Rosalia Gaglio, Raimondo Francesca, Nicola Moschetti, Giancarlo Settanni, Luca eng Research Support, Non-U.S. Gov't England 2013/09/10 Food Microbiol. 2013 Dec; 36(2):343-54. doi: 10.1016/j.fm.2013.07.003. Epub 2013 Jul 18" |
