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J Gen Microbiol

Title:		Alpha-factor enhancement of hybrid formation by protoplast fusion in the yeast Saccharomyces cerevisiae
Author(s):		Curran BP; Carter BL;
Address:
Journal Title:		J Gen Microbiol
Year:		1983
Volume:		129
Issue:		5
Page Number:		1589 - 1591
DOI:		10.1099/00221287-129-5-1589
ISSN/ISBN:		0022-1287 (Print) 0022-1287 (Linking)
Abstract:		"Two a strains of Saccharomyces cerevisiae, carrying complementary genetic markers, were arrested for 3 h with alpha-factor. These were then protoplasted, prior to fusion using polyethylene glycol. The number of viable fusion products was enhanced by a factor of 20 as compared with unarrested controls"
Keywords:		"Hybridization, Genetic/*drug effects Mating Factor Peptides/*pharmacology Protoplasts/drug effects/*physiology Saccharomyces cerevisiae/drug effects/*genetics;"
Notes:		"MedlineCurran, B P Carter, B L eng Research Support, Non-U.S. Gov't England 1983/05/01 J Gen Microbiol. 1983 May; 129(5):1589-91. doi: 10.1099/00221287-129-5-1589"