« Previous AbstractAplysia temptin - the 'glue' in the water-borne attractin pheromone complex    Next Abstract"Characterization of Aplysia Alb-1, a candidate water-borne protein pheromone released during egg laying" »

Genomics

Title:		"Gene identification and evidence for expression of G protein alpha subunits, phospholipase C, and an inositol 1,4,5-trisphosphate receptor in Aplysia californica rhinophore"
Author(s):		Cummins SF; de Vries MR; Hill KS; Boehning D; Nagle GT;
Address:		"Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555, USA. s.cummins@uq.edu.au"
Journal Title:		Genomics
Year:		2007
Volume:		20070511
Issue:		1
Page Number:		110 - 120
DOI:		10.1016/j.ygeno.2007.03.020
ISSN/ISBN:		0888-7543 (Print) 0888-7543 (Linking)
Abstract:		"In the marine mollusk Aplysia californica, waterborne protein pheromones that are released during egg laying act in concert to stimulate mate attraction. However, molecular information concerning the cellular receptors and signaling mechanisms that may be involved in waterborne peptide and protein pheromonal communication is lacking. As a first step toward examining whether members of the G protein family and phosphoinositide signaling pathway are present in the primary peripheral chemosensory organs (i.e., rhinophores), we isolated five full-length cDNA clones from an A. californica central nervous system cDNA library. These clones encoded (1) the G protein alpha subunits of the Gq, Gi, and Go families, (2) a protein with homology to phospholipase C (PLC) isoforms, and (3) an inositol 1,4,5-trisphosphate receptor (IP3R). The expression of these genes was examined using laser capture microdissection/reverse transcription-polymerase chain reaction and in situ hybridization. All of them are expressed in the rhinophore sensory epithelium, suggesting that Galphaq, Galphai, Galphao, PLC-like protein, and IP3R may be involved in waterborne protein pheromone detection in Aplysia-possibly via a phosphoinositide signaling mechanism"
Keywords:		"Amino Acid Sequence Animals Aplysia/*genetics/metabolism GTP-Binding Protein alpha Subunits/biosynthesis/*genetics *Gene Expression Regulation Inositol 1, 4, 5-Trisphosphate Receptors/biosynthesis/*genetics Microdissection Molecular Sequence Data Pheromones;neuroscience;"
Notes:		"MedlineCummins, Scott F De Vries, Melissa R Hill, Kristen S Boehning, Darren Nagle, Gregg T eng Research Support, U.S. Gov't, Non-P.H.S. 2007/05/15 Genomics. 2007 Jul; 90(1):110-20. doi: 10.1016/j.ygeno.2007.03.020. Epub 2007 May 11"