| Title: | Exhaled Volatile Organic Compounds Precedes Pulmonary Injury in a Swine Pulmonary Oxygen Toxicity Model |
| Author(s): | Cronin WA; Forbes AS; Wagner KL; Kaplan P; Cataneo R; Phillips M; Mahon R; Hall A; |
| Address: | "Walter Reed National Military Medical Center, Bethesda, MD, United States. Undersea Medicine Department, Naval Medical Research Center, Silver Spring, MD, United States. Breath Research Laboratory, Menssana Research, Inc., Newark, NJ, United States. Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States" |
| ISSN/ISBN: | 1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking) |
| Abstract: | "PURPOSE: Inspiring high partial pressure of oxygen (FiO(2) > 0.6) for a prolonged duration can lead to lung damage termed pulmonary oxygen toxicity (PO(2)T). While current practice is to limit oxygen exposure, there are clinical and military scenarios where higher FiO(2) levels and partial pressures of oxygen are required. The purpose of this study is to develop a non-invasive breath-based biomarker to detect PO(2)T prior to the onset of clinical symptoms. METHODS: Male Yorkshire swine (20-30 kg) were placed into custom airtight runs and randomized to air (0.209 FiO(2), n = 12) or oxygen (>0.95 FiO(2), n = 10) for 72 h. Breath samples, arterial blood gases, and vital signs were assessed every 12 h. After 72 h of exposure, animals were euthanized and the lungs processed for histology and wet-dry ratios. RESULTS: Swine exposed to hyperoxia developed pulmonary injury consistent with PO(2)T. Histology of oxygen-exposed swine showed pulmonary lymphatic congestion, epithelial sloughing, and neutrophil transmigration. Pulmonary injury was also evidenced by increased interstitial edema and a decreased PaO(2)/FiO(2) ratio in the oxygen group when compared to the air control group. Breath volatile organic compound (VOC) sample analysis identified six VOCs that were combined into an algorithm which generated a breath score predicting PO(2)T with a ROC/AUC curve of 0.72 defined as a of PaO(2)/FiO(2) ratio less than 350 mmHg. CONCLUSION: Exposing swine to 72 h of hyperoxia induced a pulmonary injury consistent with human clinical endpoints of PO(2)T. VOC analysis identified six VOCs in exhaled breath that preceded PO(2)T. Results show promise that a simple, non-invasive breath test could potentially predict the risk of pulmonary injury in humans exposed to high partial pressures of oxygen" |
| Keywords: | hyperoxia prediction pulmonary oxygen toxicity swine volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINECronin, William A Forbes, Angela S Wagner, Kari L Kaplan, Peter Cataneo, Renee Phillips, Michael Mahon, Richard Hall, Aaron eng Switzerland 2019/12/19 Front Physiol. 2019 Dec 3; 10:1297. doi: 10.3389/fphys.2019.01297. eCollection 2019" |
