| Title: | "Male-produced pheromone in the European woodwasp, Sirex noctilio" |
| Author(s): | Cooperband MF; Boroczky K; Hartness A; Jones TH; Zylstra KE; Tumlinson JH; Mastro VC; |
| Address: | "Otis Laboratory, USDA-APHIS-PPQ-CPHST, Buzzards Bay, MA 02542, USA. miriam.f.cooperband@aphis.usda.gov" |
| DOI: | 10.1007/s10886-012-0060-7 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "A male-produced pheromone that attracts both males and females was identified for the European woodwasp, Sirex noctilio, a serious pest of pine trees. Males displayed excitatory behaviors when placed in groups, and were attracted to the odors from males that were 2-5-d-old, but not to odors from males that were 0-1-d-old. An unsaturated short-chain alcohol, (Z)-3-decen-1-ol, was discovered in samples collected on SuperQ filters over groups of males and identified by using micro-derivatization reactions and gas chromatography coupled with mass spectrometry (GC-MS). The compound was not detected in volatile samples from females. Gas chromatography coupled electroantennographic detection (GC-EAD) of antennae from males exposed to male headspace odors produced strong antennal responses to the main peak of (Z)-3-decen-1-ol, as well as to an unknown minor component that had a similar retention time. Antennae from both males and females responded to synthetic (Z)-3-decen-1-ol. Several different synthetic candidates for the GC-EAD active minor components were selected based on GC-MS and GC-EAD responses to male headspace collections. These synthetic compounds were tested for antennal activity using GC-EAD, and those that produced strong responses were blended with the major component and tested for male attraction in the Y-tube olfactometer at different concentrations and ratios. Males tested in the Y-tube olfactometer were attracted to a synthetic blend of (Z)-3-decen-1-ol and (Z)-4-decen-1-ol at a ratio of 100:1. Whereas the addition of some suspected minor compounds reduced attraction, the addition of a third compound found in male emanations that produced strong male antennal responses, (E,E)-2,4-decadienal (at a ratio of 100:1:1), resulted in attraction of both males (Y-tube and wind tunnel) and females (wind tunnel)" |
| Keywords: | Animals Female Laboratories Male Organic Chemicals/analysis/metabolism/pharmacology Pheromones/analysis/*biosynthesis/*pharmacology Wasps/chemistry/*drug effects/*metabolism; |
| Notes: | "MedlineCooperband, Miriam F Boroczky, Katalin Hartness, Ashley Jones, Tappey H Zylstra, Kelley E Tumlinson, James H Mastro, Victor C eng 2012/01/17 J Chem Ecol. 2012 Jan; 38(1):52-62. doi: 10.1007/s10886-012-0060-7. Epub 2012 Jan 14" |
