« Previous Abstract"Discriminating Aroma Compounds in Five Cocoa Bean Genotypes from Two Brazilian States: White Kerosene-like Catongo, Red Whisky-like FL89 (Bahia), Forasteros IMC67, PA121 and P7 (Para)"    Next AbstractHypersensitivity of Arabidopsis TAXIMIN1 overexpression lines to light stress is correlated with decreased sinapoyl malate abundance and countered by the antibiotic cefotaxime »

Anal Chem

Title:		muGC x muGC: comprehensive two-dimensional gas chromatographic separations with microfabricated components
Author(s):		Collin WR; Bondy A; Paul D; Kurabayashi K; Zellers ET;
Address:		"Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109-1055, United States"
Journal Title:		Anal Chem
Year:		2015
Volume:		20150115
Issue:		3
Page Number:		1630 - 1637
DOI:		10.1021/ac5032226
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"The development and characterization of a microanalytical subsystem comprising silicon-micromachined first- and second-dimension separation columns and a silicon-micromachined thermal modulator (muTM) for comprehensive two-dimensional (i.e., muGC x muGC) separations are described. The first dimension consists of two series-coupled 3.1 cm x 3.1 cm mucolumn chips with etched channels 3 m long and 250 mum x 140 mum in cross section, wall-coated with a poly(dimethylsiloxane) (PDMS) stationary phase. The second dimension consists of a 1.2 cm x 1.2 cm mucolumn chip with an etched channel 0.5 m long and 46 mum x 150 mum in cross section wall-coated with either a trigonal tricationic room-temperature ionic liquid (RTIL) or a commercial poly(trifluoropropylmethyl siloxane) (OV-215) stationary phase. The two-stage, cryogen-free muTM consists of a Si chip containing two series-coupled, square spiral channels 4.2 cm and 2.8 cm long and 250 mum x 140 mum in cross section wall-coated with PDMS. Conventional injection methods and flame ionization detection were used. Temperature-ramped separations of a simple alkane mixture using the RTIL-coated second-dimension ((2)D) mucolumn produced reasonably good peak shapes and modulation numbers; however, strong retention of polar compounds on the RTIL-coated (2)D mucolumn led to excessively broad peaks with low (2)D resolution. Substituting OV-215 as the (2)D mucolumn stationary phase markedly improved the performance, and a structured 22 min chromatogram of a 36-component mixture spanning a vapor pressure range of 0.027 to 13 kPa was generated with modulated peak fwhm (full width at half-maximum) values ranging from 90 to 643 ms and modulation numbers of 1-6"
Keywords:		"Chromatography, Gas/*instrumentation/*methods Dimethylpolysiloxanes/*chemistry Flame Ionization Ionic Liquids Microtechnology/*instrumentation/*methods Temperature Volatile Organic Compounds/*analysis;"
Notes:		"MedlineCollin, William R Bondy, Amy Paul, Dibyadeep Kurabayashi, Katsuo Zellers, Edward T eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2014/12/24 Anal Chem. 2015 Feb 3; 87(3):1630-7. doi: 10.1021/ac5032226. Epub 2015 Jan 15"