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Cells

Title:		Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles
Author(s):		Colafarina S; Di Carlo P; Zarivi O; Aloisi M; Di Serafino A; Aruffo E; Arrizza L; Limongi T; Poma A;
Address:		"Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. Department of Psychological, Health and Territorial Sciences, University 'G. d'Annunzio' of Chieti-Pescara, 66100 Chieti, Italy. Center for Advanced Studies and Technology-CAST, University 'G. d'Annunzio' of Chieti-Pescara, 66100 Chieti, Italy. Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy"
Journal Title:		Cells
Year:		2022
Volume:		20220111
Issue:		2
Page Number:		-
DOI:		10.3390/cells11020226
ISSN/ISBN:		2073-4409 (Electronic) 2073-4409 (Linking)
Abstract:		"Indoor air pollutants (IAP), which can pose a serious risk to human health, include biological pollutants, nitric oxide (NO), nitrogen dioxide (NO(2)), volatile organic compounds (VOC), sulfur dioxide (SO(2)), carbon monoxide (CO), carbon dioxide (CO(2)), silica, metals, radon, and particulate matter (PM). The aim of our work is to conduct a multidisciplinary study of fine silica particles (<2.5 mum) in the presence or absence of ozone (O(3)), and evaluate their potential cytotoxicity using MTS, micronucleus, and the comet test in two cell lines. We analyzed A549 (human basal alveolar epithelial cell adenocarcinoma) and Hs27 (human normal fibroblasts) exposed to dynamic conditions by an IRC simulator under ozone flow (120 ppb) and in the presence of silica particles (40 mug/h). The viability of A549 and Hs27 cells at 48 and 72 h of exposure to silica or silica/ozone decreases, except at 72 h in Hs27 treated with silica/ozone. The micronucleus and comet tests showed a significant increase in the number of micronuclei and the % of DNA in the queue, compared to the control, in both lines in all treatments, even if in different cell times/types. We found that silica alone or with more O(3) causes more pronounced genotoxic effects in A549 tumor cells than in normal Hs27 fibroblasts"
Keywords:		"Adenocarcinoma/*pathology Cell Line, Tumor Comet Assay Epithelium/drug effects/*pathology Fibroblasts/drug effects/*pathology Humans Micronucleus Tests *Models, Biological Mutagens/*toxicity Ozone/*toxicity Silicon Dioxide/*toxicity A549 Hs27 Pm 2.5 genot;"
Notes:		"MedlineColafarina, Sabrina Di Carlo, Piero Zarivi, Osvaldo Aloisi, Massimo Di Serafino, Alessandra Aruffo, Eleonora Arrizza, Lorenzo Limongi, Tania Poma, Anna eng 2018-2021/University of L'Aquila/ Switzerland 2022/01/22 Cells. 2022 Jan 11; 11(2):226. doi: 10.3390/cells11020226"