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Genes Dev

Title:		The Saccharomyces retrotransposon Ty5 integrates preferentially into regions of silent chromatin at the telomeres and mating loci
Author(s):		Zou S; Ke N; Kim JM; Voytas DF;
Address:		"Department of Zoology and Genetics, Iowa State University, Ames, 50011, USA"
Journal Title:		Genes Dev
Year:		1996
Volume:		10
Issue:		5
Page Number:		634 - 645
DOI:		10.1101/gad.10.5.634
ISSN/ISBN:		0890-9369 (Print) 0890-9369 (Linking)
Abstract:		"The nonrandom integration of retrotransposons and retroviruses suggests that chromatin influences target choice. Targeted integration, in turn, likely affects genome organization. In Saccharomyces, native Ty5 retrotransposons are located near telomeres and the silent mating locus HMR. To determine whether this distribution is a consequence of targeted integration, we isolated a transposition-competent Ty5 element from S. paradoxus, a species closely related to S. cerevisiae. This Ty5 element was used to develop a transposition assay in S. cerevisiae to investigate target preference of de novo transposition events. Of 87 independent Ty5 insertions, approximately 30% were located on chromosome III, indicating this small chromosome (approximately 1/40 of the yeast genome) is a highly preferred target. Mapping of the exact location of 19 chromosome III insertions showed that 18 were within or adjacent to transcriptional silencers flanking HML and HMR or the type X subtelomeric repeat. We predict Ty5 target preference is attributable to interactions between transposition intermediates and constituents of silent chromatin assembled at these sites. Ty5 target preference extends the link between telomere structure and reverse transcription as carried out by telomerase and Drosophila retrotransposons"
Keywords:		"Amino Acid Sequence Base Sequence Blotting, Northern Chromatin/*genetics Chromosome Mapping Chromosomes, Fungal Gene Expression Regulation, Fungal Mating Factor Models, Genetic Molecular Sequence Data Peptides/*genetics RNA, Fungal/analysis RNA, Messenger;"
Notes:		"MedlineZou, S Ke, N Kim, J M Voytas, D F eng 1996/03/01 Genes Dev. 1996 Mar 1; 10(5):634-45. doi: 10.1101/gad.10.5.634"