| Title: | Reciprocal responses in the interaction between Arabidopsis and the cell-content-feeding chelicerate herbivore spider mite |
| Author(s): | Zhurov V; Navarro M; Bruinsma KA; Arbona V; Santamaria ME; Cazaux M; Wybouw N; Osborne EJ; Ens C; Rioja C; Vermeirssen V; Rubio-Somoza I; Krishna P; Diaz I; Schmid M; Gomez-Cadenas A; Van de Peer Y; Grbic M; Clark RM; Van Leeuwen T; Grbic V; |
| Address: | "Department of Biology, University of Western Ontario, London, Ontario, Canada N6A 5B7" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "Most molecular-genetic studies of plant defense responses to arthropod herbivores have focused on insects. However, plant-feeding mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g. lepidopteran larvae or aphids). The two-spotted spider mite (Tetranychus urticae) is among the most significant mite pests in agriculture, feeding on a staggering number of plant hosts. To understand the interactions between spider mite and a plant at the molecular level, we examined reciprocal genome-wide responses of mites and its host Arabidopsis (Arabidopsis thaliana). Despite differences in feeding guilds, we found that transcriptional responses of Arabidopsis to mite herbivory resembled those observed for lepidopteran herbivores. Mutant analysis of induced plant defense pathways showed functionally that only a subset of induced programs, including jasmonic acid signaling and biosynthesis of indole glucosinolates, are central to Arabidopsis's defense to mite herbivory. On the herbivore side, indole glucosinolates dramatically increased mite mortality and development times. We identified an indole glucosinolate dose-dependent increase in the number of differentially expressed mite genes belonging to pathways associated with detoxification of xenobiotics. This demonstrates that spider mite is sensitive to Arabidopsis defenses that have also been associated with the deterrence of insect herbivores that are very distantly related to chelicerates. Our findings provide molecular insights into the nature of, and response to, herbivory for a representative of a major class of arthropod herbivores" |
| Keywords: | "Animals Arabidopsis/genetics/*physiology Cyclopentanes/metabolism Female Gene Expression Profiling Gene Expression Regulation, Plant Genetic Variation Glucosinolates/metabolism Herbivory *Host-Parasite Interactions Larva Mutation Oxylipins/metabolism Plan;" |
| Notes: | "MedlineZhurov, Vladimir Navarro, Marie Bruinsma, Kristie A Arbona, Vicent Santamaria, M Estrella Cazaux, Marc Wybouw, Nicky Osborne, Edward J Ens, Cherise Rioja, Cristina Vermeirssen, Vanessa Rubio-Somoza, Ignacio Krishna, Priti Diaz, Isabel Schmid, Markus Gomez-Cadenas, Aurelio Van de Peer, Yves Grbic, Miodrag Clark, Richard M Van Leeuwen, Thomas Grbic, Vojislava eng T32 GM007464/GM/NIGMS NIH HHS/ Meta-Analysis Research Support, Non-U.S. Gov't 2013/11/29 Plant Physiol. 2014 Jan; 164(1):384-99. doi: 10.1104/pp.113.231555. Epub 2013 Nov 27" |
