| Title: | One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis |
| Author(s): | Zhu ZJ; Chen HM; Chen JJ; Yang R; Yan XJ; |
| Address: | "Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University , Ningbo, Zhejiang 315211, China. Ningbo Institute of Oceanography , Ningbo, Zhejiang 315832, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in Escherichia coli with 24.4 mg.L(-1) yield. PhLOX could catalyze the one-step bioconversion of C18-C22 fatty acids into C8-C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic and docosenoic acids than for octadecenoic acids. C20:5 was the most suitable substrate among the tested fatty acids. The C8-C9 VOCs were generated in good yields from fatty acids, e.g., 2E-nonenal from C20:4, and 2E,6Z-nonadienal from C20:5. Hydrolyzed oils were also tested as substrates. The reactions mainly generated 2E,4E-pentadienal, 2E-octenal, and 2E,4E-octadienal from hydrolyzed sunflower seed oil, corn oil, and fish oil, respectively. PhLOX showed good stability after storage at 4 degrees C for 2 weeks and broad tolerance to pH and temperature. These desirable properties of PhLOX make it a promising novel biocatalyst for the industrial production of volatile aroma compounds" |
| Keywords: | "Cloning, Molecular Corn Oil/metabolism Enzyme Stability Erucic Acids/metabolism Escherichia coli/genetics Fatty Acids/*metabolism Fatty Acids, Monounsaturated/metabolism Fish Oils/metabolism Gene Expression Hydrogen-Ion Concentration Lipoxygenase/*genetic;" |
| Notes: | "MedlineZhu, Zhu-Jun Chen, Hai-Min Chen, Juan-Juan Yang, Rui Yan, Xiao-Jun eng 2018/01/13 J Agric Food Chem. 2018 Feb 7; 66(5):1233-1241. doi: 10.1021/acs.jafc.7b05341. Epub 2018 Jan 24" |
