« Previous AbstractVolatile organic compounds (VOCs) as a rapid means for assessing the source of coprolites    Next Abstract"Representation of pheromones, interspecific signals, and plant odors in higher olfactory centers; mapping physiologically identified antennal-lobe projection neurons in the male heliothine moth" »

J Chromatogr A

Title:		Study of biodegradation products from azo dyes in fungal degradation by capillary electrophoresis/electrospray mass spectrometry
Author(s):		Zhao X; Lu Y; Phillips DR; Hwang HM; Hardin IR;
Address:		"Department of Biology, Jackson State University, Jackson, MS 39217, USA. xueheng.zhao@jsums.edu"
Journal Title:		J Chromatogr A
Year:		2007
Volume:		20061128
Issue:		1-Feb
Page Number:		217 - 224
DOI:		10.1016/j.chroma.2006.10.072
ISSN/ISBN:		0021-9673 (Print) 0021-9673 (Linking)
Abstract:		"Biodegradation products from four model sulfonated azo dyes Orange II, Acid Orange 8, Food Yellow 3, and 4-[(4-hydroxyphenyl)azo]-benzenesulfonic acid, sodium salt (4HABA), during fungal degradation were determined by capillary electrophoresis coupled with ion trap mass spectrometry (CE-MS) with electrospray ionization and a coaxial sheath flow interface. The development and optimization of this analytical method including the sheath liquid composition and flow rate, nebulizing gas flow rate, carrier electrolyte, and MS voltage are described herein. Detection of unknown biodegradation products was carried out under negative ion mode with base peak electrophorogram (BPE) or extractive ion electrophorogram (EIE) monitoring. A volatile ammonium acetate buffer (10 mM) without organic modifier and a shealth liquid made from 2-propanol and water (80:20, v/v) were suited for the separation and ESI interface. The sulfonated ion was the base peak for model azo dyes and their metabolites containing sulfonic group. Results showed that the tested azo dyes were degraded quickly in the culture of white rot fungus, Pleurotus ostreatus in 3 days with the major biodegradation products being 4-hydroxy-benzenesulfonic acid, 3-methyl-4-hydroxy-benzenesulfonic acid, benzenesulfonic acid, 1,2-naphthoquinone-6-sulfonic acid and 3-methyl-benzenesulfonic acid"
Keywords:		"Azo Compounds/*metabolism Benzenesulfonates Biodegradation, Environmental Biotransformation Coloring Agents/*metabolism Electrophoresis, Capillary/*methods Molecular Structure Pleurotus/*metabolism Reproducibility of Results Sensitivity and Specificity Sp;"
Notes:		"MedlineZhao, Xueheng Lu, Yiping Phillips, Dennis R Hwang, Huey-Min Hardin, Ian R eng Research Support, U.S. Gov't, Non-P.H.S. Netherlands 2006/11/28 J Chromatogr A. 2007 Aug 3; 1159(1-2):217-24. doi: 10.1016/j.chroma.2006.10.072. Epub 2006 Nov 28"