| Title: | Silencing of cytochrome P450 gene CYP321A1 effects tannin detoxification and metabolism in Spodoptera litura |
| Author(s): | Zhao P; Xue H; Zhu X; Wang L; Zhang K; Li D; Ji J; Niu L; Gao X; Luo J; Cui J; |
| Address: | "State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: Zhaopeng941103@163.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xuehuihzau@163.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: zhuxiangzhen318@163.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: wangli08zb@126.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: zhangkaixin@caas.cn. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: hzaulidongyang@163.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: hnnydxjc@163.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: nl19882006@126.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: 15036138389@163.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: luojunyu1818@126.com. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address: cuijinjie@126.com" |
| DOI: | 10.1016/j.ijbiomac.2021.11.144 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "Cytochrome P450 monooxygenase (P450 or CYP) plays an important role in the metabolism of insecticides and plant allelochemicals by insects. CYP321B1, a novel Spodoptera litura P450 gene, was identified and characterized. CYP321B1 contains a 1488 bp open reading frame (ORF) that encodes a 495 amino acid protein. In fourth instar larvae, the highest CYP321B1 expression levels were found in the midgut and fat body. In the tannin feeding test, tannin can significantly induce the expression of CYP321B1 in the midgut and fat body of 4th instar larvae. To verify the function of CYP321B1, RNA interference and metabolome analysis were performed. The results showed that silencing CYP321B1 significantly reduced the rate of weight gain under tannin induction. Metabolome analysis showed silencing affected 47 different metabolites, mainly involved in secondary metabolite biosynthesis and amino acid metabolism, including amino acids, lipid fatty acids, organic acids and their derivatives. Henoxyacetic acid and cysteamine are the most highly regulated metabolites, respectively. These findings demonstrate that CYP321B1 plays an important role in tannin detoxification and metabolism. Functional knowledge about metabolite detoxification genes in this major herbivorous insect pest can provide new insights into this biological process and provide new targets for agricultural pest control" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cytochrome P-450 Enzyme System/chemistry/*genetics Gene Expression Profiling Gene Expression Regulation *Gene Silencing *Inactivation, Metabolic Metabolome Metabolomics/methods Phylogeny Protein Isoforms RNA Inter;" |
| Notes: | "MedlineZhao, Peng Xue, Hui Zhu, Xiangzhen Wang, Li Zhang, Kaixin Li, Dongyang Ji, Jichao Niu, Lin Gao, Xueke Luo, Junyu Cui, Jinjie eng Netherlands 2021/11/30 Int J Biol Macromol. 2022 Jan 1; 194:895-902. doi: 10.1016/j.ijbiomac.2021.11.144. Epub 2021 Nov 27" |
