| Title: | Optimization of HS-SPME for GC-MS Analysis and Its Application in Characterization of Volatile Compounds in Sweet Potato |
| Author(s): | Zhang R; Tang C; Jiang B; Mo X; Wang Z; |
| Address: | "Crops Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Crop Genetic Improvement of Guangdong Province, Guangzhou 510640, China" |
| DOI: | 10.3390/molecules26195808 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Volatile compounds are the main chemical species determining the characteristic aroma of food. A procedure based on headspace solid-phase microextraction (HP-SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was developed to investigate the volatile compounds of sweet potato. The experimental conditions (fiber coating, incubation temperature and time, extraction time) were optimized for the extraction of volatile compounds from sweet potato. The samples incubated at 80 degrees C for 30 min and extracted at 80 degrees C by the fiber with a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) coating for 30 min gave the most effective extraction of the analytes. The optimized method was applied to study the volatile profile of four sweet potato cultivars (Anna, Jieshu95-16, Ayamursaki, and Shuangzai) with different aroma. In total, 68 compounds were identified and the dominants were aldehydes, followed by alcohols, ketones, and terpenes. Significant differences were observed among the volatile profile of four cultivars. Furthermore, each cultivar was characterized by different compounds with typical flavor. The results substantiated that the optimized HS-SPME GC-MS method could provide an efficient and convenient approach to study the flavor characteristics of sweet potato. This is the basis for studying the key aroma-active compounds and selecting odor-rich accessions, which will help in the targeted improvement of sweet potato flavor in breeding" |
| Keywords: | Gas Chromatography-Mass Spectrometry/*methods Ipomoea batatas/*metabolism Odorants/*analysis Solid Phase Microextraction/methods/*standards Taste Temperature Volatile Organic Compounds/*analysis/*isolation & purification Gc-ms Hs-spme sweet potato volatil; |
| Notes: | "MedlineZhang, Rong Tang, Chaochen Jiang, Bingzhi Mo, Xueying Wang, Zhangying eng 2019YFD1000700; 2019YFD1000701/National Key R&D Program of China/ 2020KJ111; 2021KJ111/China Agriculture Research System of MOF and MARA, the Guangdong Modern Agro-industry Technology Research System/ BZ202009/Presidential foundation of Guangdong Academy of Agricultural Sciences/ Switzerland 2021/10/14 Molecules. 2021 Sep 25; 26(19):5808. doi: 10.3390/molecules26195808" |
