« Previous AbstractThree MYB genes co-regulate the phloem-based defence against English grain aphid in wheat    Next Abstract[Pollution characterization of volatile organic compounds in ambient air of Tianjin downtown] »

Front Neural Circuits

Title:		Whole Brain Mapping of Neurons Innervating Extraorbital Lacrimal Glands in Mice and Rats of Both Genders
Author(s):		Zhai Y; Li M; Gui Z; Wang Y; Hu T; Liu Y; Xu F;
Address:		"State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Center for Magnetic Resonance, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China. Centre for Brain Research, Department of Anatomy and Medical Imaging, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. Basic Medical Laboratory, General Hospital of Central Theater Command, Wuhan, China. Hubei Key Laboratory of Central Nervous System Tumor and Intervention, Wuhan, China. College of Life Sciences, Wuhan University, Wuhan, China. Shenzhen Key Laboratory of Viral Vectors for Biomedicine, The Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, NMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products, Key Laboratory of Quality Control Technology for Virus-Based Therapeutics, Guangdong Provincial Medical Products Administration, Shenzhen, China. Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. University of Chinese Academy of Sciences, Beijing, China. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China"
Journal Title:		Front Neural Circuits
Year:		2021
Volume:		20211029
Issue:
Page Number:		768125 -
DOI:		10.3389/fncir.2021.768125
ISSN/ISBN:		1662-5110 (Electronic) 1662-5110 (Linking)
Abstract:		"The extraorbital lacrimal glands (ELGs) secret tears to maintain a homeostatic environment for ocular surfaces, and pheromones to mediate social interactions. Although its distinct gender-related differences in mice and rats have been identified, its comprehensive histology together with whole-brain neuronal network remain largely unknown. The primary objective of the present study was to investigate whether sex-specific differences take place in histological and physiological perspectives. Morphological and histological data were obtained via magnetic resonance imaging (MRI), hematoxylin-eosin (HE) staining in mice and rats of both genders. The innervating network was visualized by a pseudorabies virus (PRV) mediated retrograde trans-multi-synaptic tracing system for adult C57BL6/J mice of both genders. In terms of ELGs' anatomy, mice and rats across genders both have 7 main lobes, with one exception observed in female rats which have only 5 lobes. Both female rats and mice generally have relatively smaller shape size, absolute weight, and cell size than males. Our viral tracing revealed a similar trend of innervating patterns antero-posteriorly, but significant gender differences were also observed in the hypothalamus (HY), olfactory areas (OLF), and striatum (STR). Brain regions including piriform area (Pir), post-piriform transition area (TR), central amygdalar nucleus (CEA), medial amygdalar nucleus (MEA), lateral hypothalamic area (LHA), parasubthalamic nucleus (PSTN), pontin reticular nucleus (caudal part) (PRNc), and parabrachial nucleus, (PB) were commonly labeled. In addition, chemical isotope labeling-assisted liquid chromatography-mass spectrometry (CIL-LC-MS) and nuclear magnetic resonance spectroscopy (NMR spectroscopy) were performed to reveal the fatty acids and metabolism of the ELGs, reflecting the relationship between pheromone secretion and brain network. Overall, our results revealed basic properties and the input neural networks for ELGs in both genders of mice, providing a structural basis to analyze the diverse functions of ELGs"
Keywords:		"Animals Brain Brain Mapping Female *Lacrimal Apparatus Male Mice Mice, Inbred C57BL Neural Pathways Neurons Rats Cil-lc-ms anatomy extraorbital lacrimal gland neural circuits neural tracing;"
Notes:		"MedlineZhai, Ying Li, Min Gui, Zhu Wang, Yeli Hu, Ting Liu, Yue Xu, Fuqiang eng Research Support, Non-U.S. Gov't Switzerland 2021/11/16 Front Neural Circuits. 2021 Oct 29; 15:768125. doi: 10.3389/fncir.2021.768125. eCollection 2021"