| Title: | The SLT2(MPK1) MAP kinase is activated during periods of polarized cell growth in yeast |
| Author(s): | Zarzov P; Mazzoni C; Mann C; |
| Address: | "Departement de Biologie Cellulaire et Moleculaire, Centre d'Etudes deSaclay, France" |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "The SLT2(MPK1) mitogen-activated protein kinase signal transduction pa thway has been implicated in several biological processes in Saccharomyces cerevisiae, including the regulation of cytoskeletal and cell wall structure, polarized cell growth, and response to nutrient availability, hypo-osmotic shock and heat shock. We examined the conditions under which the SLT2 pathway is activated. We found that the SLT2 kinase is tyrosine phosphorylated and activated during periods in which yeast cells are undergoing polarized cell growth, namely during bud formation of vegetative cell division and during projection formation upon treatment with mating pheromone. BCK1(SLK1), a MEK kinase, is required for SLT2 activation in both of these situations. Upstream of BCK1(SLK1), we found that the STE20 kinase was required for SLT2 activation by mating pheromone, but was unnecessary for its activation during the vegetative cell cycle. Finally, SLT2 activation during vegetative growth was partially dependent on CDC28 in that the stimulation of SLT2 tyrosine phosphorylation was significantly reduced directly after a temperature shift in cdc28 ts mutants. Our data are consistent with a role for SLT2 in promoting polarized cell growth" |
| Keywords: | Amino Acid Sequence Calcium-Calmodulin-Dependent Protein Kinases/*metabolism *Cell Cycle DNA Primers/chemistry Enzyme Activation Fungal Proteins/*metabolism Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Mating Factor *Mitogen-Act; |
| Notes: | "MedlineZarzov, P Mazzoni, C Mann, C eng Research Support, Non-U.S. Gov't England 1996/01/02 EMBO J. 1996 Jan 2; 15(1):83-91" |
