| Title: | Genetic screens in yeast to identify mammalian nonreceptor modulators of G-protein signaling |
| Author(s): | Cismowski MJ; Takesono A; Ma C; Lizano JS; Xie X; Fuernkranz H; Lanier SM; Duzic E; |
| Address: | "Cadus Pharmaceutical Corporation, 777 Old Saw Mill River Road, Tarrytown 10591, NY, USA" |
| ISSN/ISBN: | 1087-0156 (Print) 1087-0156 (Linking) |
| Abstract: | "We describe genetic screens in Saccharomyces cerevisiae designed to identify mammalian nonreceptor modulators of G-protein signaling pathways. Strains lacking a pheromone-responsive G-protein coupled receptor and expressing a mammalian-yeast Galpha hybrid protein were made conditional for growth upon either pheromone pathway activation (activator screen) or pheromone pathway inactivation (inhibitor screen). Mammalian cDNAs that conferred plasmid-dependent growth under restrictive conditions were identified. One of the cDNAs identified from the activator screen, a human Ras-related G protein that we term AGS1 (for activator of G-protein signaling), appears to function by facilitating guanosine triphosphate (GTP) exchange on the heterotrimeric Galpha. A cDNA product identified from the inhibitor screen encodes a previously identified regulator of G-protein signaling, human RGS5" |
| Keywords: | "Cloning, Molecular/*methods DNA, Complementary/genetics Epistasis, Genetic GTP-Binding Proteins/genetics/*isolation & purification/*metabolism Gene Library Genetic Engineering Humans Phenotype Pheromones/metabolism Protein Binding Saccharomyces cerevisiae;" |
| Notes: | "MedlineCismowski, M J Takesono, A Ma, C Lizano, J S Xie, X Fuernkranz, H Lanier, S M Duzic, E eng R01-NS24821/NS/NINDS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1999/09/03 Nat Biotechnol. 1999 Sep; 17(9):878-83. doi: 10.1038/12867" |
