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Insect Biochem Mol Biol

Title:		"Sex pheromone receptors of the light brown apple moth, Epiphyas postvittana, support a second major pheromone receptor clade within the Lepidoptera"
Author(s):		Yuvaraj JK; Jordan MD; Zhang DD; Andersson MN; Lofstedt C; Newcomb RD; Corcoran JA;
Address:		"Department of Biology, Lund University, Lund, Sweden. Electronic address: jothi_kumar.yuvaraj@biol.lu.se. The New Zealand Institute for Plant and Food Research Ltd, Auckland, New Zealand. Electronic address: melissa.jordan@plantandfood.co.nz. Department of Biology, Lund University, Lund, Sweden. Electronic address: dan-dan.zhang@biol.lu.se. Department of Biology, Lund University, Lund, Sweden. Electronic address: martin_n.andersson@biol.lu.se. Department of Biology, Lund University, Lund, Sweden. Electronic address: christer.lofstedt@biol.lu.se. The New Zealand Institute for Plant and Food Research Ltd, Auckland, New Zealand. Electronic address: richard.newcomb@plantandfood.co.nz. Department of Biology, Lund University, Lund, Sweden; The New Zealand Institute for Plant and Food Research Ltd, Auckland, New Zealand. Electronic address: jacob.corcoran@usda.gov"
Journal Title:		Insect Biochem Mol Biol
Year:		2022
Volume:		20211229
Issue:
Page Number:		103708 -
DOI:		10.1016/j.ibmb.2021.103708
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Sex pheromones facilitate species-specific sex communication within the Lepidoptera. They are detected by specialised pheromone receptors (PRs), most of which to date fall into a single monophyletic receptor lineage (frequently referred to as 'the PR clade') within the odorant receptor (OR) family. Here we investigated PRs of the invasive horticultural pest, Epiphyas postvittana, commonly known as the light brown apple moth. Ten candidate PRs were selected, based on their male-biased expression in antennae or their relationship to the PR clade, for functional assessment in both HEK293 cells and Xenopus oocytes. Of these, six ORs responded to compounds that include components of the E. postvittana ('Epos') sex pheromone blend or compounds that antagonise sex pheromone attraction. In phylogenies, four of the characterised receptors (EposOR1, 6, 7 and 45) fall within the PR clade and two other male-biased receptors (EposOR30 and 34) group together well outside the PR clade. This new clade of pheromone receptors includes the receptor for (E)-11-tetradecenyl acetate (EposOR30), which is the main component of the sex pheromone blend for this species. Interestingly, receptors of the two clades do not segregate by preference for compounds associated with behavioural response (agonist or antagonist), isomer type (E or Z) or functional group (alcohol or acetate), with examples of each scattered across both clades. Phylogenetic comparison with PRs from other species supports the existence of a second major clade of lepidopteran ORs including, EposOR30 and 34, that has been co-opted into sex pheromone detection in the Lepidoptera. This second clade of sex pheromone receptors has an origin that likely predates the split between the major lepidopteran families"
Keywords:		"Animals Female HEK293 Cells Humans Male Moths/*genetics Phylogeny Receptors, Pheromone/classification/*genetics Sex Attractants/*genetics Lbam Lepidoptera PR clade Sex pheromone Xenopus oocytes;"
Notes:		"MedlineYuvaraj, Jothi Kumar Jordan, Melissa D Zhang, Dan-Dan Andersson, Martin N Lofstedt, Christer Newcomb, Richard D Corcoran, Jacob A eng Research Support, Non-U.S. Gov't England 2022/01/02 Insect Biochem Mol Biol. 2022 Feb; 141:103708. doi: 10.1016/j.ibmb.2021.103708. Epub 2021 Dec 29"