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BMC Genomics

Title:		Transcriptome profiling provides new insights into the formation of floral scent in Hedychium coronarium
Author(s):		Yue Y; Yu R; Fan Y;
Address:		"The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. yueyuechong@stu.scau.edu.cn. College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China. rcyu@scau.edu.cn. The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. fanyanping@scau.edu.cn"
Journal Title:		BMC Genomics
Year:		2015
Volume:		20150619
Issue:		1
Page Number:		470 -
DOI:		10.1186/s12864-015-1653-7
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: Hedychium coronarium is a popular ornamental plant in tropical and subtropical regions because its flowers not only possess intense and inviting fragrance but also enjoy elegant shape. The fragrance results from volatile terpenes and benzenoids presented in the floral scent profile. However, in this species, even in monocots, little is known about the underlying molecular mechanism of floral scent production. RESULTS: Using Illumina platform, approximately 81 million high-quality reads were obtained from a pooled cDNA library. The de novo assembly resulted in a transcriptome with 65,591 unigenes, 50.90% of which were annotated using public databases. Digital gene expression (DGE) profiling analysis revealed 7,796 differential expression genes (DEGs) during petal development. GO term classification and KEGG pathway analysis indicated that the levels of transcripts changed significantly in 'metabolic process', including 'terpenoid biosynthetic process'. Through a systematic analysis, 35 and 33 candidate genes might be involved in the biosynthesis of floral volatile terpenes and benzenoids, respectively. Among them, flower-specific HcDXS2A, HcGPPS, HcTPSs, HcCNL and HcBCMT1 might play critical roles in regulating the formation of floral fragrance through DGE profiling coupled with floral volatile profiling analyses. In vitro characterization showed that HcTPS6 was capable of generating beta-farnesene as its main product. In the transcriptome, 1,741 transcription factors (TFs) were identified and 474 TFs showed differential expression during petal development. It is supposed that two R2R3-MYBs with flower-specific and developmental expression might be involved in the scent production. CONCLUSIONS: The novel transcriptome and DGE profiling provide an important resource for functional genomics studies and give us a dynamic view of biological process during petal development in H. coronarium. These data lay the basis for elucidating the molecular mechanism of floral scent formation and regulation in monocot. The results also provide the opportunities for genetic modification of floral scent profile in Hedychium"
Keywords:		"Flowers/*genetics Gene Expression Profiling/methods Gene Expression Regulation, Plant/*genetics Gene Library Genes, Plant/genetics Plant Proteins/genetics Sesquiterpenes/metabolism Terpenes/metabolism Transcription Factors/genetics Transcriptome/*genetics;"
Notes:		"MedlineYue, Yuechong Yu, Rangcai Fan, Yanping eng Research Support, Non-U.S. Gov't England 2015/06/19 BMC Genomics. 2015 Jun 19; 16(1):470. doi: 10.1186/s12864-015-1653-7"