| Title: | Transcriptome Signatures in Pseudomonas simiae WCS417 Shed Light on Role of Root-Secreted Coumarins in Arabidopsis-Mutualist Communication |
| Author(s): | Yu K; Stringlis IA; van Bentum S; de Jonge R; Snoek BL; Pieterse CMJ; Bakker P; Berendsen RL; |
| Address: | "Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. State Key Laboratory of Crop Stress Adaptation and Improvement, Henan University, Kaifeng 475004, China. Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Ghent, Belgium. Theoretical Biology & Bioinformatics, Department of Biology, Science4Life, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands" |
| DOI: | 10.3390/microorganisms9030575 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Pseudomonas simiae WCS417 is a root-colonizing bacterium with well-established plant-beneficial effects. Upon colonization of Arabidopsis roots, WCS417 evades local root immune responses while triggering an induced systemic resistance (ISR) in the leaves. The early onset of ISR in roots shows similarities with the iron deficiency response, as both responses are associated with the production and secretion of coumarins. Coumarins can mobilize iron from the soil environment and have a selective antimicrobial activity that impacts microbiome assembly in the rhizosphere. Being highly coumarin-tolerant, WCS417 induces the secretion of these phenolic compounds, likely to improve its own niche establishment, while providing growth and immunity benefits for the host in return. To investigate the possible signaling function of coumarins in the mutualistic Arabidopsis-WCS417 interaction, we analyzed the transcriptome of WCS417 growing in root exudates of coumarin-producing Arabidopsis Col-0 and the coumarin-biosynthesis mutant f6'h1. We found that coumarins in F6'H1-dependent root exudates significantly affected the expression of 439 bacterial genes (8% of the bacterial genome). Of those, genes with functions related to transport and metabolism of carbohydrates, amino acids, and nucleotides were induced, whereas genes with functions related to cell motility, the bacterial mobilome, and energy production and conversion were repressed. Strikingly, most genes related to flagellar biosynthesis were down-regulated by F6'H1-dependent root exudates and we found that application of selected coumarins reduces bacterial motility. These findings suggest that coumarins' function in the rhizosphere as semiochemicals in the communication between the roots and WCS417. Collectively, our results provide important novel leads for future functional analysis of molecular processes in the establishment of plant-mutualist interactions" |
| Keywords: | coumarins induced systemic resistance iron deficiency plant-beneficial rhizobacteria transcriptomics; |
| Notes: | "PubMed-not-MEDLINEYu, Ke Stringlis, Ioannis A van Bentum, Sietske de Jonge, Ronnie Snoek, Basten L Pieterse, Corne M J Bakker, Peter A H M Berendsen, Roeland L eng 269072/ERC_/European Research Council/International FWO 12B8116RN/Fonds Wetenschappelijk Onderzoek/ fellowship to Ke Yu/China Scholarship Council/ Switzerland 2021/04/04 Microorganisms. 2021 Mar 11; 9(3):575. doi: 10.3390/microorganisms9030575" |
