| Title: | "Pimenta dioica and Pimenta racemosa: GC-based metabolomics for the assessment of seasonal and organ variation in their volatile components, in silico and in vitro cytotoxic activity estimation" |
| Author(s): | Youssef FS; Labib RM; Gad HA; Eid S; Ashour ML; Eid HH; |
| Address: | "Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, 11566, Cairo, Egypt. Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia. Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, 11566, Cairo, Egypt and Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 21442, Jeddah, Saudi Arabia. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, 11562, Cairo, Egypt. hanaaeideg@gmail.com" |
| ISSN/ISBN: | 2042-650X (Electronic) 2042-6496 (Linking) |
| Abstract: | "Volatile constituents isolated from the stems (S) and leaves (L) of Pimenta dioica (PD) and Pimenta racemosa (PR) during the four seasons were analyzed using GLC/FID (Gas liquid chromatography - flame ionization detector) and GLC/MS (Gas liquid chromatography - mass spectrometry). Eighty-nine compounds were identified in all samples, in which oxygenated monoterpene represented by eugenol was the major constituent in PDS-S3 (autumn) (88.71%) and PDS-S2 (summer) (88.41%). Discrimination between P. dioica and P. racemosa leaves and stems in different seasons was achieved by applying chemometrics analysis comprising Principal Component Analysis (PCA) and Hierarchal Cluster Analysis (HCA). For P. dioica, they were partially segregated where leaves collected from spring and autumn were superimposed, and similarly for P. dioica stems collected in summer and autumn. For P. racemosa leaves, the PCA score plot showed that all seasons were completely segregated from each other, with the winter and autumn samples being in very close distance to each other. P. racemosa stems collected in autumn and spring exhibited significant variation, as they were completely detached from each other. Moreover, summer and winter fell in a near distance to each other. An in vitro cell viability assay was done to evaluate the variation in the cytotoxicity of the isolated essential oils against breast (MCF-7), hepatic (HepG-2), and cervical (HeLa-2) cancer cell lines using the MTT assay. The maximum cytotoxic effect was observed by PDL against HeLa, HepG-2 and MCF-7 cells with IC50 values equal to 122.1, 139.6, and 178.7 mug mL-1, respectively. An in silico study was done to assess the cytotoxic effect of the major compounds detected in the oils by determining their inhibitory effect on human DNA topoisomerase II (TOP-2), human cyclin-dependent kinase 2 (CDK-2) and matrix metalloproteinase 13 (MMP-13). o-Cymene followed by eugenol showed the highest fitting with all of the examined proteins approaching doxorubicin. It can be concluded that GC coupled with chemometrics provide a strong tool for the discrimination of samples, while Pimenta could afford a natural drug that could alleviate cancer" |
| Keywords: | "Cell Line Computer Simulation Gas Chromatography-Mass Spectrometry Humans *Metabolomics Myrtaceae/*chemistry Neoplasms/drug therapy Oils, Volatile/chemistry/pharmacology Pharmaceutical Preparations Pimenta/*chemistry Plant Leaves Plant Oils *Seasons Volat;" |
| Notes: | "MedlineYoussef, Fadia S Labib, Rola M Gad, Haidy A Eid, SafaaY Ashour, Mohamed L Eid, Hanaa H eng England 2021/05/15 Food Funct. 2021 Jun 21; 12(12):5247-5259. doi: 10.1039/d1fo00408e" |
