| Title: | PI3K/Akt Activated by GPR30 and Src Regulates 17beta-Estradiol-Induced Cultured Immature Boar Sertoli Cells Proliferation |
| Author(s): | Yang WR; Zhu FW; Zhang JJ; Wang Y; Zhang JH; Lu C; Wang XZ; |
| Address: | "1 College of Animal Science and Technology, Southwest University, Chongqing, China. 2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China. 3 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China. 4 Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China" |
| ISSN/ISBN: | 1933-7205 (Electronic) 1933-7191 (Linking) |
| Abstract: | "Sertoli cell (SC) is a key element in the process of spermatogenesis. Accumulating research show that estrogen plays an important role in regulating boar SC proliferation. However, it is unclear whether phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B (PI3K/Akt) is involved in this process. In the present study, the role of PI3K/Akt on the 17beta-estradiol-induced piglet SC proliferation was explored. In addition, we also explained the roles of G-protein-coupled estrogen receptor (GPR30) and Sarcoma protein (Src) in this process. Our study demonstrated that, 17beta-estradiol induced activation of PI3K in a time-dependent manner. Both G-15 (an antagonist of GPR30, 0.1 mumol/L) and PP2 (an inhibitor of Src, 2.0 mumol/L) inhibited 17beta-estradiol-induced activation of PI3K, reduced SC proliferation, and decreased messenger RNA (mRNA) and protein expression of S-phase kinase-associated protein 2 (Skp2). We also found that 17beta-estradiol induced activation of Akt in a time-dependent manner. Both LY294002 (an inhibitor of PI3K) and 10-DEBC (an inhibitor of Akt) significantly reduced 17beta-estradiol-induced SC proliferation and reduced mRNA and protein expression of Skp2. In addition, LY294002 inhibited 17beta-estradiol-induced activation of Akt. The results indicated that 17beta-estradiol regulates SC proliferation by activating PI3K/Akt. Both GPR30 and Src are involved in 17beta-estradiol-induced phosphorylation of PI3K/Akt. Activation of PI3K/Akt enhances the expression of Skp2, which promotes SC proliferation" |
| Keywords: | 17beta-estradiol Gpr30 PI3K/Akt Sertoli cell Src; |
| Notes: | "PubMed-not-MEDLINEYang, Wei-Rong Zhu, Feng-Wei Zhang, Jiao-Jiao Wang, Yi Zhang, Jia-Hua Lu, Cheng Wang, Xian-Zhong eng 2016/05/26 Reprod Sci. 2017 Jan; 24(1):57-66. doi: 10.1177/1933719116649696. Epub 2016 Sep 27" |
