| Title: | Targeted mutagenesis of an odorant receptor co-receptor using TALEN in Ostrinia furnacalis |
| Author(s): | Yang B; Fujii T; Ishikawa Y; Matsuo T; |
| Address: | "Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: matsuo@utlae.org" |
| DOI: | 10.1016/j.ibmb.2015.12.003 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Genome editing using transcription activator-like effector nuclease (TALEN) has been applied for various model organisms but not yet for agricultural pest insects. In this study, TALEN-mediated mutagenesis of the gene encoding odorant receptor co-receptor (Orco) of an important agricultural pest Ostrinia furnacalis (OfurOrco) was carried out. Of the two pairs of TALEN constructs designed, one generated somatic and germline mutations at rates of 70.8% and 20.8%, respectively. Physiological and behavioral analyses using a gas chromatograph-electroantennographic detector system and a wind tunnel, respectively, revealed that antennal responses to sex pheromone components were decreased to trace levels, and behavioral responses were abolished in OfurOrco mutants. This study demonstrated that TALEN-mediated mutagenesis is applicable to pest insects, and these results will open the way for a better understanding of chemosensory systems in wild insects" |
| Keywords: | "Animals Endonucleases/*metabolism Germ-Line Mutation Lepidoptera/*metabolism *Mutagenesis Receptors, Odorant/*genetics/metabolism Genome editing Knock out Lepidoptera Mutant Non-model insect;" |
| Notes: | "MedlineYang, Bin Fujii, Takeshi Ishikawa, Yukio Matsuo, Takashi eng England 2015/12/23 Insect Biochem Mol Biol. 2016 Mar; 70:53-9. doi: 10.1016/j.ibmb.2015.12.003. Epub 2015 Dec 12" |
