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J Cell Biol

Title:		"Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)"
Author(s):		Yamamoto A; Guacci V; Koshland D;
Address:		"Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA"
Journal Title:		J Cell Biol
Year:		1996
Volume:		133
Issue:		1
Page Number:		99 - 110
DOI:		10.1083/jcb.133.1.99
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"We report the isolation and characterization of pds1 mutants in Saccharomyces cerevisiae. The initial pds1-1 allele was identified by its inviability after transient exposure to microtubule inhibitors and its precocious dissociation of sister chromatids in the presence of these microtubule inhibitors. These findings suggest that pds1 mutants might be defective in anaphase arrest that normally is imposed by a spindle-damage checkpoint. To further examine a role for Pds1p in anaphase arrest, we compared the cell cycle arrest of pds1 mutants and PDS1 cells after: (a) the inactivation of Cdc16p or Cdc23p, two proteins that are required for the degradation of mitotic cyclins and are putative components of the yeast anaphase promoting complex (APC); (b) the inactivation of Cdc20p, another protein implicated in the degradation of mitotic cyclins; and (c) the inactivation of Cdc13 protein or gamma irradiation, two circumstances that induce a DNA-damage checkpoint. Under all these conditions, anaphase is inhibited in PDS1 cells but not in pds1 mutants. From these results we suggest that Pds1 protein is an anaphase inhibitor in PDS1 cells but not in pds1 mutants. From these results we suggest that Pds1 protein is an anaphase inhibitor that plays a critical role in the control of anaphase by both APC and checkpoints. We also show that pds1 mutants exit mitosis and initiate new rounds of cell division after gamma irradiation and Cdc13p inactivation but no after nocodazole-treatment or inactivation of Cdc16p, Cdc20p or Cdc23p function. Therefore, in the DNA-damage checkpoint, Pds1p is required for the inhibition of cytokinesis and DNA replication as well as anaphase. The role of Pds1 protein in anaphase inhibition and general cell cycle regulation is discussed"
Keywords:		"Anaphase/*physiology Cell Cycle Cell Cycle Proteins/physiology Cell Division Chromatids/*physiology DNA Damage DNA, Fungal/analysis/radiation effects Fungal Proteins/*physiology Gamma Rays Mating Factor Microtubules/drug effects Mitosis/physiology Mutatio;"
Notes:		"MedlineYamamoto, A Guacci, V Koshland, D eng GM41718/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1996/04/01 J Cell Biol. 1996 Apr; 133(1):99-110. doi: 10.1083/jcb.133.1.99"