| Title: | Total synthesis of the lipopeptide a-mating factor of Saccharomyces cerevisiae |
| Author(s): | Xue CB; Caldwell GA; Becker JM; Naider F; |
| Address: | "Department of Chemistry, College of Staten Island, New York" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/0006-291x(89)91989-x |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "The a-mating factor of Saccharomyces cerevisiae was synthesized using both solution phase and solid phase strategies. Structure of the final peptide was confirmed using amino acid analysis, fast atom bombardment mass spectroscopy and 400 MHz proton NMR. The synthetic farnesylated dodecapeptide, YIIKGVFWDPAC (S-farnesyl) OCH3, exhibited chromatographic and spectroscopic properties identical to the natural pheromone and had significant biological activity at nanomolar concentrations" |
| Keywords: | "Acetates Acetic Acid Amino Acid Sequence Chromatography, High Pressure Liquid Fungal Proteins/*chemical synthesis/pharmacology Mass Spectrometry Mating Factor Methanol Molecular Sequence Data Peptides/*chemical synthesis/isolation & purification/pharmacol;" |
| Notes: | "MedlineXue, C B Caldwell, G A Becker, J M Naider, F eng GM 22086/GM/NIGMS NIH HHS/ GM 22087/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1989/07/14 Biochem Biophys Res Commun. 1989 Jul 14; 162(1):253-7. doi: 10.1016/0006-291x(89)91989-x" |
