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J Biol Chem

Title:		"Stage-dependent and temperature-controlled expression of the gene encoding the precursor protein of diapause hormone and pheromone biosynthesis activating neuropeptide in the silkworm, Bombyx mori"
Author(s):		Xu WH; Sato Y; Ikeda M; Yamashita O;
Address:		"Laboratory of Sericultural Science and Entomoresources, School of Agricultural Sciences, Nagoya University, Japan"
Journal Title:		J Biol Chem
Year:		1995
Volume:		270
Issue:		8
Page Number:		3804 - 3808
DOI:		10.1074/jbc.270.8.3804
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Embryonic diapause and sex pheromone biosynthesis in the silkworm, Bombyx mori, are, respectively, induced by diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN), which are produced in the subesophageal ganglion from a common polyprotein precursor (DH-PBAN precursor) encoded by a single gene (DH-PBAN gene). Using DH-PBAN cDNA as a probe, we quantitatively measured DH-PBAN mRNA content throughout embryonic and postembryonic development and observed the effects of incubation temperature, which is a key factor for determination of diapause, on DH-PBAN gene expression. The silkworm, which is programmed to lay diapause eggs by being incubated at 25 degrees C, showed peaks of DH-PBAN mRNA content at five different stages throughout the life cycle: at the late embryonic stage, at the middle of the fourth and the fifth larval instars, and at early and late stages of pupal-adult development. In the non-diapause type silkworms programmed by a 15 degrees C incubation, only the last peak of DH-PBAN mRNA in pupal-adult development was found, and the other peaks were absent. Furthermore, interruption of the incubation period at 25 degrees C by incubation at 15 degrees C decreased both DH-PBAN mRNA content in mature embryos and in subesophageal ganglia of day 3 pupae and the incidence of diapause eggs. Thus, there were two types of regulatory mechanisms for DH-PBAN gene expression. One is a temperature-controlled expression that is responsible for diapause induction, and the other is a temperature-independent, stage-dependent expression related to pheromone production"
Keywords:		"Animals Base Sequence Bombyx/embryology/*genetics DNA Probes *Gene Expression Regulation, Developmental Molecular Sequence Data Neuropeptides/*genetics RNA, Messenger/genetics/metabolism Temperature;"
Notes:		"MedlineXu, W H Sato, Y Ikeda, M Yamashita, O eng Research Support, Non-U.S. Gov't 1995/02/24 J Biol Chem. 1995 Feb 24; 270(8):3804-8. doi: 10.1074/jbc.270.8.3804"