| Title: | Testing the effect of individual scent compounds on pollinator attraction in nature using quasi-isogenic Capsella lines |
| Author(s): | Xiong YZ; Kappel C; Hagemann L; Jantzen F; Wozniak N; Sicard A; Huang SQ; Lenhard M; |
| Address: | "School of Life Sciences, Central China Normal University, Luoyu Avenue 152, Wuhan, 430079, China. Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, Potsdam-Golm, 14476, Germany. Applied Zoology /Animal Ecology, Freie Universitat Berlin, Haderslebener Str. 9, Berlin, 12163, Germany. Department of Plant Biology, Uppsala Biocenter, BOX 7080, Uppsala, 750 07, Sweden" |
| ISSN/ISBN: | 1537-2197 (Electronic) 0002-9122 (Linking) |
| Abstract: | "PREMISE OF THE STUDY: Floral scent, usually consisting of multiple compounds, is a complex trait and its role in pollinator attraction has received increasing attention. However, it is difficult to disentangle the effect of individual floral scent compounds due to the complexity of isolating the effect of single compounds by traditional methods. METHODS: Using available quasi-isogenic lines (qILs) generated as part of the original mapping of two floral scent volatile-related loci, CNL1 (benzaldehyde) and TPS2 (beta-ocimene), in Capsella, we have generated four genotypes that should only systematically differ in these two compounds. Plants of the four genotypes were introduced into a common garden outside the natural range of C. rubella or C. grandiflora, with individuals of a self-compatible C. grandiflora line as pollen donors, whose different genetic background facilitates the detection of outcrossing events. Visitors to flowers of all five genotypes were compared, and the seeds set during the common-garden period were collected for high-throughput amplicon-based sequencing to estimate their outcrossing rates. KEY RESULTS: Benzaldehyde and beta-ocimene emissions were detected in the floral scent of corresponding genotypes. While some pollinator groups showed specific visitation preferences depending on scent compounds, the outcrossing rates in seeds did not vary among the four scent-manipulated genotypes. CONCLUSIONS: The successful construction of scent-manipulated Capsella materials using qILs provides a powerful system to study the ecological effects of individual floral scent compounds under largely natural environments. In Capsella, individual BAld and beta-ocimene emission may act as attractants for different types of pollinators. This article is protected by copyright. All rights reserved" |
| Keywords: | Brassicaceae Capsella benzaldehyde floral scent pollinator attraction quasi-isogenic line beta-ocimene; |
| Notes: | "PublisherXiong, Ying-Ze Kappel, Christian Hagemann, Laura Jantzen, Friederike Wozniak, Natalia Sicard, Adrien Huang, Shuang-Quan Lenhard, Michael eng 2023/09/04 Am J Bot. 2023 Sep 4. doi: 10.1002/ajb2.16237" |
