« Previous AbstractUnifloral Autumn Heather Honey from Indigenous Greek Erica manipuliflora Salisb.: SPME/GC-MS Characterization of the Volatile Fraction and Optimization of the Isolation Parameters    Next AbstractWhen being alone is enough: noncanonical functions of canonical bacterial quorum-sensing systems »

Pharm Biol

Title:		"Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil"
Author(s):		Xavier JA; Valentim IB; Camatari FOS; de Almeida AMM; Goulart HF; Ferro JNS; Barreto EO; Cavalcanti BC; Bottoli CBG; Goulart MOF;
Address:		"a Instituto de Quimica e Biotecnologia , Universidade Federal de Alagoas (UFAL) , Maceio , AL , Brazil. b Instituto Federal de Educacao , Ciencia e Tecnologia de Alagoas (IFAL), Rua Mizael Domingues , Maceio , AL , Brazil. c Empresa Baiana de Desenvolvimento Agricola S.A (EBDA) , Salvador , BA , Brazil. d Laboratorio de Pesquisas em Recursos Naturais, Centro de Ciencias Agrarias (CECA), UFAL , Rio Largo , AL , Brazil. e Laboratorio de Biologia Celular, UFAL , Maceio , AL , Brazil. f Departamento de Fisiologia e Farmacologia , Laboratorio Nacional de Oncologia Experimental, Universidade Federal do Ceara , Fortaleza , CE , Brazil. g Instituto de Quimica , Universidade de Campinas , Campinas , SP , Brazil"
Journal Title:		Pharm Biol
Year:		2017
Volume:		55
Issue:		1
Page Number:		1884 - 1893
DOI:		10.1080/13880209.2017.1340962
ISSN/ISBN:		1744-5116 (Electronic) 1388-0209 (Print) 1388-0209 (Linking)
Abstract:		"CONTEXT: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities. OBJECTIVE: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. MATERIAL AND METHODS: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH(*), FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0.04-25 mug/mL concentrations, for 72 h. The determination of primary phenols by ultra high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) and volatile organic compounds content by gas chromatography-mass spectrometry (GC-MS) were also performed. RESULTS: The EEP polar fractions exhibited up to 90% protection against lipid peroxidation. The IC(50) value for anti-glycation activity of EEP was between 16.5 and 19.2 mug/mL, close to aminoguanidine (IC(50) = 7.7 mug/mL). The use of UHPLC-MS/MS and GC-MS allowed the identification of 12 bioactive phenols in the EEP and 24 volatile compounds, all already reported. CONCLUSIONS: The samples present good antioxidant/anti-glycation/cytotoxic activities and a plethora of biologically active compounds. These results suggest a potential role of propolis in targeting ageing and diseases associated with oxidative and carbonylic stress, aggregating value to them"
Keywords:		Animals Antineoplastic Agents/adverse effects/chemistry/isolation & purification/*pharmacology Antioxidants/adverse effects/chemistry/isolation & purification/*pharmacology Biological Products/adverse effects/chemistry/isolation & purification/*pharmacolo;
Notes:		"MedlineXavier, Jadriane de Almeida Valentim, Iara Barros Camatari, Fabiana O S de Almeida, Alberto M M Goulart, Henrique Fonseca Ferro, Jamylle Nunes de Souza Barreto, Emiliano de Oliveira Cavalcanti, Bruno Coelho Bottoli, Carla B G Goulart, Marilia Oliveira Fonseca eng Comparative Study England 2017/06/21 Pharm Biol. 2017 Dec; 55(1):1884-1893. doi: 10.1080/13880209.2017.1340962"