| Title: | Transcriptome analysis of antennal cytochrome P450s and their transcriptional responses to plant and locust volatiles in Locusta migratoria |
| Author(s): | Wu H; Liu Y; Shi X; Zhang X; Ye C; Zhu KY; Zhu F; Zhang J; Ma E; |
| Address: | "Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: wuhaihua04@sxu.edu.cn. Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China. Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China. Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA. Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA. Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: zjz@sxu.edu.cn. Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. Electronic address: maenbo2003@sxu.edu.cn" |
| DOI: | 10.1016/j.ijbiomac.2020.01.309 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "Cytochrome P450 monooxygenases (P450s) constitute a large superfamily of heme-thiolate proteins that are involved in the biosynthesis or degradation of endogenous compounds and detoxification of exogenous chemicals. It has been reported that P450s could serve as odorant-degrading enzymes (ODEs) to inactivate odorants to avoid saturating the antennae. However, there is little information about P450s in the antennae of Locusta migratoria. In the current work, we conducted an antenna transcriptome analysis and identified 92 P450s, including 68 full-length and 24 partial sequences. Phylogenetic analysis showed that 68 full-length P450s were grouped into four clans: CYP2, CYP3, CYP4, and mitochondria clans. Tissue, stage, and sex-dependent expressions of these 68 P450s were investigated. The results showed that 4 P450s were antenna-specific, whereas others were antenna-rich but also expressed in other tissues, implying their various potential roles in the antennae. In addition, the responses of seven selected P450s to five gramineous plant volatiles and four locust volatiles were determined. CYP6MU1 could be induced by almost all compounds tested, suggesting its important roles in odorant processing. Different P450s exhibited diverse responses to odorants, indicating that specific regulation of P450 expression by odorants might modulate the sensitivity of the olfactory responses to various chemicals" |
| Keywords: | Animals Cytochrome P-450 Enzyme System/classification/*genetics/*metabolism Cytochrome P450 Family 2/metabolism Cytochrome P450 Family 3/metabolism Cytochrome P450 Family 4/metabolism Gene Expression Profiling/*methods Gene Expression Regulation Inactivat; |
| Notes: | "MedlineWu, Haihua Liu, Yongmei Shi, Xuekai Zhang, Xueyao Ye, Changlu Zhu, Kun Yan Zhu, Fang Zhang, Jianzhen Ma, Enbo eng Netherlands 2020/02/06 Int J Biol Macromol. 2020 Apr 15; 149:741-753. doi: 10.1016/j.ijbiomac.2020.01.309. Epub 2020 Feb 1" |
