| Title: | Two Antenna-Enriched Odorant Binding Proteins in Dioryctria abietella Tuned to General Odorants and Insecticides |
| Author(s): | Wu C; Yin N; Guo Y; Wang Z; Liu N; |
| Address: | "Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "The management of forest pests has become a significant challenge, particularly for wood borers, because they spend most of the time in the trunks or cones. The coneworm, Dioryctria abietella, is a representative of cone borers as its larvae feed on the cones of Pinaceae plants. The molecular mechanisms underlying the interactions between this species and host plants or habitats can assist in developing strategies for pest control. In this study, we extended the expression profiles of 32 odorant binding proteins (OBPs) in the reproductive tissues of D. abietella, revealing the detectable transcription of 29 genes. Using two DabiOBPs highly expressed in antennae (DabiOBP5 and DabiOBP14) as targets, six compounds with high affinities (dissociation constants < 13 muM) were identified through a reverse chemical ecology strategy, including insecticides widely used for the control of lepidopteran pests. Of these compounds, a floral volatile beta-ionone and a pear-produced ester ethyl-(2E,4Z)-decadienoate may serve as behaviorally active compounds in D. abietella. The strong binding of DabiOBPs to insecticides suggested their involvement in insecticide resistance, reflecting sophisticated detoxification mechanisms of this moth. In the molecular simulations, DabiOBP14 possessed stronger interactions with the six ligands compared to DabiOBP5, in which a few key residues within the binding pockets were involved in the formation of hydrogen bonds. This study provides some valuable reference active compounds for the development of lures or repellents in D. abietella and unravels the putative roles of two antenna-dominant DabiOBPs in the perception of plant-derived odorants and insecticides" |
| Keywords: | Dioryctria abietella insecticide resistance ligand-binding assay molecular simulation odorant binding protein plant odorant reproduction; |
| Notes: | "PubMed-not-MEDLINEWu, Chun Yin, Ningna Guo, Yuruo Wang, Zhengquan Liu, Naiyong eng 202001AT070100/Yunnan Fundamental Research Project/ 31960115/National Natural Science Foundation of China/ YNWR_ QNBJ_2019_057/Yunnan Provincial Support Plan for the Cultivation of High-level Talents Young Top-notch Talents/ Switzerland 2022/12/24 Insects. 2022 Dec 12; 13(12):1145. doi: 10.3390/insects13121145" |
