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Microb Drug Resist

Title:		Plasmid-mediated vanB glycopeptide resistance in enterococci
Author(s):		Woodford N; Morrison D; Johnson AP; Bateman AC; Hastings JG; Elliott TS; Cookson B;
Address:		"Laboratory of Hospital Infection, Central Public Health Laboratory, London, UK"
Journal Title:		Microb Drug Resist
Year:		1995
Volume:		1
Issue:		3
Page Number:		235 - 240
DOI:		10.1089/mdr.1995.1.235
ISSN/ISBN:		1076-6294 (Print) 1076-6294 (Linking)
Abstract:		"Enterococcus faecium, which was highly resistant to vancomycin (MIC 256 mg/liter), but susceptible to teicoplanin (MIC 2 mg/liter), caused two distinct episodes of infection on a renal unit in the United Kingdom. Pulsed field gel electrophoresis (PFGE) indicated that a single strain caused the first episode, while the second episode, which occurred 1 year later, involved multiple strains, all of which were distinct from the original strain. Vancomycin resistance in all but one of these strains was mediated by transferable plasmids that carried the vanB glycopeptide resistance gene. Transfer either of resistance plasmids or the vanB resistance determinant itself to different strains occurred during the second episode. Plasmid-mediated vanB resistance has not been widely documented. A retrospective study of a reference collection revealed two other vanB-encoding plasmids from an E. faecalis and an E. faecium referred from two further UK centers. Although restriction analysis indicated no similarity between the plasmids from the three different centers, all contained a 2.1-kb EcoRV fragment that hybridized with a probe for the vanB gene. This suggests that there has been dissemination of a conserved glycopeptide resistance determinant, of which vanB is a part"
Keywords:		"Anti-Bacterial Agents/*pharmacology Bacterial Proteins/*genetics Conjugation, Genetic Cross Infection/microbiology DNA Probes Drug Resistance, Microbial/genetics Electrophoresis, Polyacrylamide Gel Enterococcus faecium/*drug effects/*genetics Genes, Bacte;"
Notes:		"MedlineWoodford, N Morrison, D Johnson, A P Bateman, A C Hastings, J G Elliott, T S Cookson, B eng 1995/10/01 Microb Drug Resist. 1995 Fall; 1(3):235-40. doi: 10.1089/mdr.1995.1.235"