| Title: | The Saccharomyces cerevisiae pheromone-response is a metabolically active stationary phase for bio-production |
| Author(s): | Williams TC; Peng B; Vickers CE; Nielsen LK; |
| Address: | "Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St. Lucia, QLD 4072, Australia" |
| DOI: | 10.1016/j.meteno.2016.05.001 |
| ISSN/ISBN: | 2214-0301 (Electronic) 2214-0301 (Linking) |
| Abstract: | "The growth characteristics and underlying metabolism of microbial production hosts are critical to the productivity of metabolically engineered pathways. Production in parallel with growth often leads to biomass/bio-product competition for carbon. The growth arrest phenotype associated with the Saccharomyces cerevisiae pheromone-response is potentially an attractive production phase because it offers the possibility of decoupling production from population growth. However, little is known about the metabolic phenotype associated with the pheromone-response, which has not been tested for suitability as a production phase. Analysis of extracellular metabolite fluxes, available transcriptomic data, and heterologous compound production (para-hydroxybenzoic acid) demonstrate that a highly active and distinct metabolism underlies the pheromone-response. These results indicate that the pheromone-response is a suitable production phase, and that it may be useful for informing synthetic biology design principles for engineering productive stationary phase phenotypes" |
| Keywords: | "Dynamic regulation GFP, Green Fluorescent Protein MAPK, Mitogen Activated Protein Kinase Mating Metabolic engineering Metabolic productivity PHBA, para-hydroxybenzoic acid Shikimate Stationary phase Synthetic biology Yeast;" |
| Notes: | "PubMed-not-MEDLINEWilliams, Thomas C Peng, Bingyin Vickers, Claudia E Nielsen, Lars K eng Netherlands 2016/05/11 Metab Eng Commun. 2016 May 11; 3:142-152. doi: 10.1016/j.meteno.2016.05.001. eCollection 2016 Dec" |
