| Title: | "Separation, identification, and design of alpha-glucosidase inhibitory peptides based on the molecular mechanism from Paeonia ostii 'Feng Dan' seed protein" |
| Author(s): | Wei R; Lin L; Li T; Li C; Chen B; Shen Y; |
| Address: | "Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, China" |
| ISSN/ISBN: | 1750-3841 (Electronic) 0022-1147 (Linking) |
| Abstract: | "Peptides are considered promising sources of nutraceuticals. In this study, a mixture of peptides was prepared from Paeonia ostii 'Feng Dan' seed meal protein by continuous enzymolysis. Successive separation and purification procedures, including ultrafiltration and reversed-phase high-performance liquid chromatography (RP-HPLC), were performed, and six novel peptides were identified by liquid chromatography-electrospray ionization source-mass spectrometry/mass spectrometry (LC-ESI-MS/MS). In an in vitro antidiabetic activity test, Tyr-Phe-Phe-Met exhibited stronger alpha-glucosidase inhibitory activity (48.17 +/- 3.34% at 1 mg/mL) than the other peptides. Docking studies of this peptide into the active site of alpha-glucosidase showed that the formation of hydrogen bonds could be critical for the enzymatic trapping of inhibitory peptides. Furthermore, two novel peptides, Phe-Phe-Phe-Met (IC(50) = 245.46 +/- 44.01 microM) and Tyr-Tyr-Phe-Met (IC(50) = 306.71 +/- 48.17 microM), with improved alpha-glucosidase inhibitory activity, were designed based on molecular docking. Therefore, the seed meal of Paeonia ostii could be considered a functional food ingredient for the management of hyperglycemia, and three novel peptides were identified as alpha-glucosidase inhibitors" |
| Keywords: | *alpha-Glucosidases *Paeonia Tandem Mass Spectrometry Molecular Docking Simulation Peptides/chemistry Seeds antidiabetic peptide enzymatic hydrolysis identification mechanism of inhibition molecular docking; |
| Notes: | "MedlineWei, Ruiting Lin, Like Li, Tingting Li, Cong Chen, Bang Shen, Yehua eng 2019YFD1002400/National Key Research and Development Projects of China/ 2018ZDXM-NY-087/Shaanxi Key Research and Development Program/ 2019TSLNY03-02/Shaanxi Key Research and Development Program/ 2018-2-21/Yulin Science and Technology Planning Project/ Amygdalus Pedunculata Engineering Technology Research Center of the State Forestry Administration/ 2022/10/08 J Food Sci. 2022 Nov; 87(11):4892-4904. doi: 10.1111/1750-3841.16340. Epub 2022 Oct 7" |
