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Insect Mol Biol

Title:		Two classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatiles
Author(s):		Wei HS; Qin JH; Cao YZ; Li KB; Yin J;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China"
Journal Title:		Insect Mol Biol
Year:		2021
Volume:		20210417
Issue:		4
Page Number:		390 - 399
DOI:		10.1111/imb.12703
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"Insects possess a fairly sophisticated olfactory system in their antennae to detect odorants essential for their survival and reproduction. Among them, insect first perceives odour sources by odorant-binding proteins (OBPs) to locate host-plants. Methyl salicylate, (Z)-3-hexenyl acetate and dibutyl phthalate are major volatile components of Ulmus pumila and Ricinus communis and elicit strong responses of the scarab beetle Holotrichia oblita adults. However, olfactory perception of the scarab beetle to these odorant compounds is unclear. In the current study, we cloned the OBP6 and OBP7 of H. oblita. The expression pattern shows that the two genes were highly expressed in the antennae of female beetles. Binding assays verified that the HoblOBP6 had a better binding affinity to methyl salicylate, and so did HoblOBP7 to (Z)-3-hexenyl acetate and dibutyl phthalate. The effect on the responses of female beetles to the three compounds was decreased significantly after these two genes were silenced by RNA interference. These results indicate that HoblOBP6 and HoblOBP7 are essential for female H. oblita perception of methyl salicylate, (Z)-3-hexenyl acetate and dibutyl phthalate. Our study provides important insights into the olfactory mechanism of female H. oblita to ester plant volatiles and could facilitate the development of potential pest control strategies in the field"
Keywords:		"Animals Arthropod Antennae/metabolism *Coleoptera/metabolism/physiology Dibutyl Phthalate/metabolism Esters/metabolism Female Genes, Insect Insect Control Insect Proteins/genetics/metabolism Odorants Olfactory Perception Plants/metabolism *Receptors, Odor;"
Notes:		"MedlineWei, H-S Qin, J-H Cao, Y-Z Li, K-B Yin, J eng Research Support, Non-U.S. Gov't England 2021/04/07 Insect Mol Biol. 2021 Aug; 30(4):390-399. doi: 10.1111/imb.12703. Epub 2021 Apr 17"