| Title: | Sensilla localization and sex pheromone recognition of odorant binding protein OBP4 in the mirid plant bug Adelphocoris lineolatus (Goeze) |
| Author(s): | Wang Q; Wang Q; Li H; Sun L; Zhang D; Zhang Y; |
| Address: | "College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou 311300, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Key Laboratory of Tea Quality and Safety Control, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou 311300, China. Key Laboratory of Tea Quality and Safety Control, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou 311300, China. Electronic address: zhangdayu@zafu.edu.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn" |
| DOI: | 10.1016/j.jinsphys.2020.104012 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Linking) |
| Abstract: | "Pheromone binding proteins (PBPs) are well studied in lepidopteran moths and are considered to be crucial in detection of sex pheromones as well as some green leaf volatiles. In contrast, evidence that PBPs interact with sex pheromones of hemipteran species is not available. The mirid plant bug, Adelphocoris lineolatus (Goeze), is a notorious hemipteran pest that uses two butyrate esters, trans-2-hexenyl butyrate (E2HB) and hexyl butyrate (HB), and one hexenoic aldehyde trans-4-oxo-2-hexenal (E4O2H), as sex pheromones. In the present study, we report on an odorant binding protein, AlinOBP4, with particular focus on its potential physiological roles in the detection of A. lineolatus sex pheromone components. Phylogenetic analyses indicated that AlinOBP4 and two mirid orthologs clustered in a general phylogenetic clade with the lepidopteran ABX OBPs, the fly LUSH and the OBP83a/b subfamily. Cellular localization by fluorescence in situ hybridization and immunolabeling further demonstrated that AlinOBP4 was strongly expressed in the multiporous sensilla trichodea (str) and middle long sensilla basiconica (mlsba) of male A. lineolatus adults, suggesting a key role associated with sex pheromone and odorant detection. A ligand binding assay revealed that recombinant AlinOBP4 protein highly bound not only to the sex pheromone components E4O2H but also to some host plant volatiles. These findings together with the evidence of insect PBPs available in the literature support the view that AlinOBP4 is involved in sex pheromone detection in male A. lineolatus and provide foundational information for further elucidating the molecular mechanisms of chemosensory based mating behavior in hemipteran mirid bugs" |
| Keywords: | "Animals Female Gene Expression Genes, Insect Heteroptera/*physiology/ultrastructure Immunohistochemistry In Situ Hybridization, Fluorescence Insect Proteins/metabolism Male Phylogeny Plants/metabolism *Receptors, Odorant/genetics/metabolism Recombinant Pr;" |
| Notes: | "MedlineWang, Qian Wang, Qi Li, Hongyue Sun, Liang Zhang, Dayu Zhang, Yongjun eng Research Support, Non-U.S. Gov't England 2020/01/09 J Insect Physiol. 2020 Feb-Mar; 121:104012. doi: 10.1016/j.jinsphys.2020.104012. Epub 2020 Jan 3" |
