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PLoS One

Title:		Identification and characterization of an antennae-specific aldehyde oxidase from the navel orangeworm
Author(s):		Choo YM; Pelletier J; Atungulu E; Leal WS;
Address:		"Honorary Maeda-Duffey Laboratory, University of California Davis, Davis, California, United States of America"
Journal Title:		PLoS One
Year:		2013
Volume:		20130624
Issue:		6
Page Number:		e67794 -
DOI:		10.1371/journal.pone.0067794
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Antennae-specific odorant-degrading enzymes (ODEs) are postulated to inactivate odorant molecules after they convey their signal. Different classes of insect ODEs are specific to esters, alcohols, and aldehydes--the major functional groups of female-produced, hydrophobic sex pheromones from moth species. Esterases that rapidly inactive acetate and other esters have been well-studied, but less is known about aldehyde oxidases (AOXs). Here we report cloning of an aldehyde oxidase, AtraAOX2, from the antennae of the navel orangeworm (NOW), Amyelois transitella, and the first activity characterization of a recombinant insect AOX. AtraAOX2 gene spans 3,813 bp and encodes a protein with 1,270 amino acid residues. AtraAOX2 cDNA was expressed in baculovirus-infected insect Sf21 cells as a approximately 280 kDa homodimer with 140 kDa subunits. Recombinant AtraAOX2 degraded Z11Z13-16Ald and plant volatile aldehydes as substrates. However, as expected for aldehyde oxidases, recombinant AtraAOX2 did not show specificity for Z11Z13-16Ald, the main constituent of the sex pheromone, but showed high activity for plant volatile aldehydes. Our data suggest AtraAOX2 might be involved in degradation of a diversity of aldehydes including sex pheromones, plant-derived semiochemicals, and chemical cues for oviposition sites. Additionally, AtraAOX2 could protect the insect's olfactory system from xenobiotics, including pesticides that might reach the sensillar lymph surrounding the olfactory receptor neurons"
Keywords:		1-Propanol/metabolism Aldehyde Oxidase/antagonists & inhibitors/chemistry/genetics/*metabolism Amino Acid Sequence Animals Arthropod Antennae/drug effects/*enzymology Bombyx/enzymology Enzyme Activation/drug effects Enzyme Inhibitors/pharmacology Female G;
Notes:		"MedlineChoo, Young-Moo Pelletier, Julien Atungulu, Elizabeth Leal, Walter S eng Research Support, U.S. Gov't, Non-P.H.S. 2013/07/05 PLoS One. 2013 Jun 24; 8(6):e67794. doi: 10.1371/journal.pone.0067794. Print 2013"