| Title: | Volatile-Mediated Inhibitory Activity of Rhizobacteria as a Result of Multiple Factors Interaction: The Case of Lysobacter capsici AZ78 |
| Author(s): | Vlassi A; Nesler A; Parich A; Puopolo G; Schuhmacher R; |
| Address: | "Department of Agrobiotechnology (IFA-Tulln), Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences Vienna (BOKU), 3430 Tulln, Austria. Bi-PA nv (Biological Products for Agriculture), 1840 Londerzeel, Belgium. Center of Agriculture, Food, Environment, University of Trento, 38098 San Michele all'Adige, Italy. Research and Innovation Centre, Department of Sustainable Agro-Ecosystems and Bioresources, Fondazione Edmund Mach, 38098 San Michele all'Adige, Italy" |
| DOI: | 10.3390/microorganisms8111761 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Plant beneficial rhizobacteria may antagonize soilborne plant pathogens by producing a vast array of volatile organic compounds (VOCs). The production of these compounds depends on the medium composition used for bacterial cell growth. Accordingly, Lysobacter capsici AZ78 (AZ78) grown on a protein-rich medium was previously found to emit volatile pyrazines with toxic activity against soilborne phypathogenic fungi and oomycetes. However, the discrepancy between the quantity of pyrazines in the gaseous phase and the minimum quantity needed to achieve inhibition of plant pathogens observed, lead us to further investigate the volatile-mediated inhibitory activity of AZ78. Here, we show that, besides VOCs, AZ78 cells produce ammonia in increased amounts when a protein-rich medium is used for bacterial growth. The production of this volatile compound caused the alkalinization of the physically separated culture medium where Rhizoctonia solani was inoculated subsequently. Results achieved in this work clearly demonstrate that VOC, ammonia and the growth medium alkalinization contribute to the overall inhibitory activity of AZ78 against R. solani. Thus, our findings suggest that the volatile-mediated inhibitory activity of rhizobacteria in protein-rich substrates can be regarded as a result of multiple factors interaction, rather than exclusively VOCs production" |
| Keywords: | Lysobacter capsici AZ78 Rhizoctonia solani Voc ammonia protein-rich medium rhizobacteria substrate pH; |
| Notes: | "PubMed-not-MEDLINEVlassi, Anthi Nesler, Andrea Parich, Alexandra Puopolo, Gerardo Schuhmacher, Rainer eng 722642/H2020-EU/ Switzerland 2020/11/14 Microorganisms. 2020 Nov 9; 8(11):1761. doi: 10.3390/microorganisms8111761" |
