| Title: | Whole-cell bacterial biosensor for volatile detection from Pectobacterium-infected potatoes enables early identification of potato tuber soft rot disease |
| Author(s): | Veltman B; Harpaz D; Melamed S; Tietel Z; Tsror L; Eltzov E; |
| Address: | "Institute of Postharvest and Food Science, Department of Postharvest Science, Volcani Institute, Agricultural Research Organization, Rishon LeZion, 7505101, Israel; Institute of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel. Electronic address: boris.veltman@mail.huji.ac.il. Institute of Postharvest and Food Science, Department of Postharvest Science, Volcani Institute, Agricultural Research Organization, Rishon LeZion, 7505101, Israel; Institute of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel. Electronic address: dorin.harpaz@mail.huji.ac.il. Department of Food Science, Gilat Research Center, Agricultural Research Organization, M.P, Negev, 8531100, Israel. Electronic address: saritm@volcani.agri.gov.il. Department of Food Science, Gilat Research Center, Agricultural Research Organization, M.P, Negev, 8531100, Israel. Electronic address: tietel@agri.gov.il. Department of Plant Pathology and Weed Research, Institute of Plant Protection, Gilat Research Center, Agricultural Research Organization, M.P, Negev, 8531100, Israel. Electronic address: tsror@agri.gov.il. Institute of Postharvest and Food Science, Department of Postharvest Science, Volcani Institute, Agricultural Research Organization, Rishon LeZion, 7505101, Israel. Electronic address: eltzov@volcani.agri.gov.il" |
| DOI: | 10.1016/j.talanta.2022.123545 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Linking) |
| Abstract: | "Half of the harvested food is lost due to rots caused by microorganisms. Plants emit various volatile organic compounds (VOCs) into their surrounding environment, and the VOC profiles of healthy crops are altered upon infection. In this study, a whole-cell bacterial biosensor was used for the early identification of potato tuber soft rot disease caused by the pectinolytic bacteria Pectobacterium in potato tubers. The detection is based on monitoring the luminescent responses of the bacteria panel to changes in the VOC profile following inoculation. First, gas chromatography-mass spectrometry (GC-MS) was used to specify the differences between the VOC patterns of the inoculated and non-inoculated potato tubers during early infection. Five VOCs were identified, 1-octanol, phenylethyl alcohol, 2-ethyl hexanol, nonanal, and 1-octen-3-ol. Then, the infection was detected by the bioreporter bacterial panel, firstly measured in a 96-well plate in solution, and then also tested in potato plugs and validated in whole tubers. Examination of the bacterial panel responses showed an extensive cytotoxic effect over the testing period, as seen by the elevated induction factor (IF) values in the bacterial strain TV1061 after exposure to both potato plugs and whole tubers. Moreover, quorum sensing influences were also observed by the elevated IF values in the bacterial strain K802NR. The developed whole-cell biosensor system based on bacterial detection will allow more efficient crop management during postharvest, storage, and transport of crops, to reduce food losses" |
| Keywords: | *Biosensing Techniques *Pectobacterium Plant Diseases *Solanum tuberosum *Volatile Organic Compounds Bioluminescent bacterial panel Cytotoxicity and quorum sensing effects Pectobacterium Potato tuber soft rot Volatile organic compounds (VOCs) Whole-cell b; |
| Notes: | "MedlineVeltman, Boris Harpaz, Dorin Melamed, Sarit Tietel, Zipora Tsror, Leah Eltzov, Evgeni eng Netherlands 2022/05/22 Talanta. 2022 Sep 1; 247:123545. doi: 10.1016/j.talanta.2022.123545. Epub 2022 May 14" |
