| Title: | Regulators of floral fragrance production and their target genes in petunia are not exclusively active in the epidermal cells of petals |
| Author(s): | Van Moerkercke A; Galvan-Ampudia CS; Verdonk JC; Haring MA; Schuurink RC; |
| Address: | "Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking) |
| Abstract: | "In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it is exclusively active in these cells remains to be proven. Cell-specific transcription factors activating these genes will determine in which cells they are expressed. In petunia, the transcription factor EMISSION OF BENZENOIDS II (EOBII) activates the ODORANT1 (ODO1) promoter and the promoter of the biosynthetic gene isoeugenol synthase (IGS). The regulator ODO1 in turn activates the promoter of the shikimate gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Here the identification of a new target gene of ODO1, encoding an ABC transporter localized on the plasma membrane, PhABCG1, which is co-expressed with ODO1, is described. PhABCG1 expression is up-regulated in petals overexpressing ODO1 through activation of the PhABCG1 promoter. Interestingly, the ODO1, PhABCG1, and IGS promoters were active in petunia protoplasts originating from both epidermal and mesophyll cell layers of the petal, suggesting that the volatile phenylpropanoid/benzenoid pathway in petunia is active in these different cell types. Since volatile release occurs from epidermal cells, trafficking of (volatile) compounds between cell layers must be involved, but the exact function of PhABCG1 remains to be resolved" |
| Keywords: | "ATP-Binding Cassette Transporters/chemistry/genetics/metabolism Amino Acid Sequence Cell Membrane/metabolism Flowers/*cytology/*genetics *Gene Expression Regulation, Plant Genes, Plant/*genetics Intracellular Space/metabolism Molecular Sequence Data Molec;" |
| Notes: | "MedlineVan Moerkercke, Alex Galvan-Ampudia, Carlos S Verdonk, Julian C Haring, Michel A Schuurink, Robert C eng England 2012/02/22 J Exp Bot. 2012 May; 63(8):3157-71. doi: 10.1093/jxb/ers034. Epub 2012 Feb 15" |
