| Title: | The transcription factors Tec1 and Ste12 interact with coregulators Msa1 and Msa2 to activate adhesion and multicellular development |
| Author(s): | van der Felden J; Weisser S; Bruckner S; Lenz P; Mosch HU; |
| Address: | "Department of Genetics, Philipps-Universitat, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany. Department of Physics, Philipps-Universitat, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany. Department of Genetics, Philipps-Universitat, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany moesch@staff.uni-marburg.de" |
| ISSN/ISBN: | 1098-5549 (Electronic) 0270-7306 (Print) 0270-7306 (Linking) |
| Abstract: | "In Saccharomyces cerevisiae and related yeast species, the TEA transcription factor Tec1, together with a second transcription factor, Ste12, controls development, including cell adhesion and filament formation. Tec1-Ste12 complexes control target genes through Tec1 binding sites (TEA consensus sequences [TCSs]) that can be further combined with Ste12 binding sites (pheromone response elements [PREs]) for cooperative DNA binding. The activity of Tec1-Ste12 complexes is known to be under negative control of the Dig1 and Dig2 (Dig1/2) transcriptional corepressors that confer regulation by upstream signaling pathways. Here, we found that Tec1 and Ste12 can associate with the transcriptional coregulators Msa1 and Msa2 (Msa1/2), which were previously found to associate with the cell cycle transcription factor complexes SBF (Swi4/Swi6 cell cycle box binding factor) and MBF (Mbp1/Swi6 cell cycle box binding factor) to control G1-specific transcription. We further show that Tec1-Ste12-Msa1/2 complexes (i) do not contain Swi4 or Mbp1, (ii) assemble at single TCSs or combined TCS-PREs in vitro, and (iii) coregulate genes involved in adhesive and filamentous growth by direct promoter binding in vivo. Finally, we found that, in contrast to Dig proteins, Msa1/2 seem to act as coactivators that enhance the transcriptional activity of Tec1-Ste12. Taken together, our findings add an additional layer of complexity to our understanding of the control mechanisms exerted by the evolutionarily conserved TEA domain and Ste12-like transcription factors" |
| Keywords: | "Cell Adhesion Cell Cycle Proteins/metabolism DNA, Fungal/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation, Fungal Models, Biological Promoter Regions, Genetic/genetics Protein Binding Saccharomyces cerevisiae/*cytology/genetics/*growt;" |
| Notes: | "Medlinevan der Felden, Julia Weisser, Sarah Bruckner, Stefan Lenz, Peter Mosch, Hans-Ulrich eng Research Support, Non-U.S. Gov't 2014/04/16 Mol Cell Biol. 2014 Jun; 34(12):2283-93. doi: 10.1128/MCB.01599-13. Epub 2014 Apr 14" |
