| Title: | A profile of volatile organic compounds in breath discriminates COPD patients from controls |
| Author(s): | Van Berkel JJ; Dallinga JW; Moller GM; Godschalk RW; Moonen EJ; Wouters EF; van Schooten FJ; |
| Address: | "Department of Health Risk Analysis and Toxicology, Faculty of Health, Medicine and Life Sciences, Research Institute NUTRIM, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands" |
| DOI: | 10.1016/j.rmed.2009.10.018 |
| ISSN/ISBN: | 1532-3064 (Electronic) 0954-6111 (Linking) |
| Abstract: | "BACKGROUND: Chronic obstructive pulmonary disease (COPD) is an inflammatory condition characterized by oxidative stress and the formation of volatile organic compounds (VOCs) secreted via the lungs. We recently developed a methodological approach able to identify profiles of VOCs in breath unique for patient groups. Here we applied this recently developed methodology regarding diagnosis of COPD patients. METHODS: Fifty COPD patients and 29 controls provided their breath and VOCs were analyzed by gas chromatography-mass spectrometry to identify relevant VOCs. An additional 16 COPD patients and 16 controls were sampled in order to validate the model, and 15 steroid naive COPD patients were sampled to determine whether steroid use affects performance. FINDINGS: 1179 different VOCs were detected, of which 13 were sufficient to correctly classify all 79 subjects. Six of these 13 VOCs classified 92% of the subjects correctly (sensitivity: 98%, specificity: 88%) and correctly classified 29 of 32 subjects (sensitivity: 100%, specificity: 81%) from the independent validation population. Fourteen out of 15 steroid naive COPD patients were correctly classified thus excluding treatment influences. INTERPRETATION: This is the first study distinguishing COPD subjects from controls solely based on the presence of VOCs in breath. Analysis of VOCs might be highly relevant for diagnosis of COPD" |
| Keywords: | "Adrenal Cortex Hormones/therapeutic use Aged Biomarkers/analysis Breath Tests/methods Case-Control Studies Exhalation Female Gas Chromatography-Mass Spectrometry/methods Humans Male Middle Aged Oxidative Stress/physiology Pulmonary Disease, Chronic Obstru;" |
| Notes: | "MedlineVan Berkel, J J B N Dallinga, J W Moller, G M Godschalk, R W L Moonen, E J Wouters, E F M Van Schooten, F J eng Research Support, Non-U.S. Gov't Validation Study England 2009/11/13 Respir Med. 2010 Apr; 104(4):557-63. doi: 10.1016/j.rmed.2009.10.018. Epub 2009 Nov 10" |
