| Title: | The yeast repeated element sigma contains a hormone-inducible promoter |
| Author(s): | Van Arsdell SW; Stetler GL; Thorner J; |
| DOI: | 10.1128/mcb.7.2.749-759.1987 |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "A genomic clone (lambda ScG7) from Saccharomyces cerevisiae encoded a 650-nucleotide poly(A)-containing [poly(A)+] RNA that was about 50 times more abundant in MATa cells that had been exposed to the peptide pheromone alpha-factor than in untreated cells. This RNA was transcribed from a cluster of repetitive sequences: both intact and truncated delta and sigma elements adjacent to a tRNATrp gene. Strand-specific probes indicated that this RNA initiated within an intact sigma element and contained sigma sequences at its 5' end. MATa cells produced two other prominent poly(A)+ RNAs (500 and 5,300 bases) in response to alpha-factor that were homologous to the same strand of sigma but transcribed from other locations in the genome. Induction of the sigma-related transcripts was rapid, was not blocked by inhibition of protein synthesis, required a functional receptor (STE2 gene product), and hence appeared to be a primary response to pheromone. Pulse-labeling confirmed that accumulation of sigma RNA following alpha-factor administration was accounted for by an increase in its rate of transcription. The sigma RNAs also were induced in MAT alpha cells that had been treated with a-factor, but were not present at significant levels in MATa/MAT alpha diploids. In MATa cells transformed with a plasmid in which the lambda ScG7 sigma element was inserted just upstream of a gene coding for the intracellular form of invertase (SUC2) lacking its own promoter, a new poly(A)+ RNA (2.2 kilobases) appeared in response to alpha-factor that hybridized to both sigma and SUC2 probes, and intracellular invertase activity was elevated about 10-fold within 30 min. Primer extension showed that transcription from the hybrid gene initiated exclusively within the sigma sequence (117 nucleotides from the 3' end of the element)" |
| Keywords: | "Chromosome Mapping Gene Expression Regulation Glycoside Hydrolases/genetics Mating Factor Peptides/*physiology *Promoter Regions, Genetic RNA, Fungal/genetics Recombinant Fusion Proteins/genetics *Repetitive Sequences, Nucleic Acid Saccharomyces cerevisia;" |
| Notes: | "MedlineVan Arsdell, S W Stetler, G L Thorner, J eng GM-09499/GM/NIGMS NIH HHS/ GM-21841/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1987/02/01 Mol Cell Biol. 1987 Feb; 7(2):749-59. doi: 10.1128/mcb.7.2.749-759.1987" |
